Purification and Antimicrobial Assay of an Antimicrobial Protein from a Biocontrol Bacterium Strain K2-1 against Aquatic Pathogens  

生防细菌K2-1抗菌蛋白纯化及其拮抗水产病原菌分析（英文）

作　　者：唐旭[1] 刘源森[1] 林凌[1] 黄仕新 方卫东[2] 徐长安[1] 

机构地区：[1]国家海洋局第三海洋研究所,福建厦门361005 [2]集美大学水产学院,福建厦门361005

出　　处：《Agricultural Science & Technology》2015年第8期1582-1583,1675,共3页农业科学与技术（英文版）

基　　金：Supported by Science and Technology Plan Project of Fujian Province(2013Y0063);Xiamen South Ocean Research Centre Project(13GZP002NF08)~~

摘　　要：[Objective] The aim of this study was to purify an antimicrobial protein from a biocontrol bacterium strain K2-1 and analyze its antimicrobial activity in vitro against some typical aquatic pathogens. [Method] The antimicrobial protein was ob- tained by using ammonium sulfate precipitation and Sephadex chromatography combined with hot water bath. The antimicrobial assay was conducted by means of agar diffusion technique, using Vibrio alginolyticus, Aeromonas hydrophila, Aeromonas. Sobria, Pseudomonas fluorescens, Vibrio Parahaemolyticus, Vibrio har- veyi and Vibrio anguillarum as test bacteria. [Result] Antimicrobial protein APK2 can be derived from fermentation broth of strain K2-1 and purified to the chromatogra- phy pure level by the methods provided, the final yield of the antimicrobial compo- nent is approximately 0.08%. This antimicrobial protein had a strong antimicrobial activity against the growth of most those bacteria. [Conclusion] The results show that APK2 could be a potential alternative to replace chemical antimicrobial agent in the control and prevention of aquatic diseases.[目的]纯化源自生防细菌K2-1的抗菌蛋白并进行抗菌蛋白于体外对主要水产病原菌的拮抗分析。[方法]利用硫酸铵沉淀,而后透析,获得抗菌蛋白粗品,应用凝胶色谱结合水浴,进一步对抗菌蛋白粗品进行纯化;采用琼脂扩散法,进行抗菌蛋白体外拮抗溶藻弧菌、嗜水气单胞菌、温和气单胞菌、荧光假单胞菌、副溶血弧菌、哈维氏弧菌及鳗弧菌实验,分析其抗菌活性。[结果]应用上述分离纯化方法,获得色谱纯的抗菌蛋白APK2,过程回收率为0.08%,拮抗实验表明,该抗菌蛋白对多数应试病原菌具有较强的抑制作用。[结论]抗菌蛋白APK2有可能作为化学抗生素的替代品,用于水产细菌病的绿色防治。

关 键 词：Antimicrobial Protein PURIFICATION Aquatic Pathogen 

分 类 号：S941.4[农业科学—水产养殖]