Interspecific Cross Compatibility of Rhododendron in Changbai Mountain  

长白山杜鹃花属种间杂交亲和性的研究（英文）

作　　者：李西时 巴春影[1] 曹后男[1] 宗成文[1] 曲柳[1] 张吉子 

机构地区：[1]延边大学农学院,吉林延边133002 [2]延边农业科学院,吉林延边133002

出　　处：《Agricultural Science & Technology》2015年第8期1617-1620,共4页农业科学与技术（英文版）

基　　金：Supported by Students’ Innovation and Entrepreneurship Training Program of Yanbian University in 2015(ydbksky2015252)~~

摘　　要：In order to explore the interspecific cross compatibility of Rhododendron and the feasibility of interspecific cross in germplasrn innovation, reciprocal crosses among five species of Rhododendron in Changbai Mountain were made, In addition, the hybrid embryos were cultured in vitro. The results showed that the species of Rhododendron in Changbai Mountain had significant differences in reciprocal cross compatibility. When Rh. chrysanthum Pall. was used as female parent, it showed high cross compatibilities with Rh. mucronulatum Turcz. （92.55%）, Rh. mucronulamm Turcz. f, album Nakai （94.14%） and Yabuli Rhododendron （92.42%）, and no signifi- cant differences were found in fruit setting rate by serf-poUination. The germination rates of hybrid embryos were 13.60%, 11.59% and 4.12%, respectively. The hybrid seedlings had weak growth vigor and albino status and were trended to be aborted. After 2-3 generations of subculture, the growth vigor of hybrid seedlings were re- covered gradually, and their differentiation rate was also significantly higher than that of Rh. chrysanthum Pall. seedlings. But the hybrid seedlings were still yellowish green. In reciprocal crosses, the fruit setting rates were significantly reduced. The combination with the highest fruit setting rate （36.51%） was Yabuli Rhododendron x Rh chrysanthum Pall The fruit setting rates of reciprocal crosses between Rh chrysan- thum Pall and Rh. mucronulatum Turcz. and Rh.： chrysanthum Pall. and Rh. schlip- penbachii Maxim were 6.52% and 0, respectively. However, the hybrid embryo＇s germination rate （39,81%） of Yabuli Rhododendron x Rh. chrysanthum Pall. was significantly higher than that of their original cross. Moreover, the hybrid seedlings grew strongly and could be directly transplanted without subculture.为了探索长白山杜鹃花属种间杂交的亲和性及种间杂交在种质创新中的可行性。以5种长白山杜鹃花属植物为试材，进行种闯正厦杂交，并对杂种胚进行离体培养。结果表明，杜鹃花属种间正反杂交亲和力有显著差异，以牛皮杜鹃为母本，与迎红杜鹃、白花迎红杜鹃、亚布力杜鹃杂交有较高的亲和健。与母本的自然授粉坐果率无显著差异。分别为92．55锈、94．14％、92．42％，杂种胚的萌发率分别为13．60％、11．59％、4．12％：杂种苗生长势微弱，呈白化状态，有败育迹象，经继代堵养2～3次后，生长势得到一定的恢复，且分化率明显高于牛皮杜鹃种胚苗，但植株的颜色仍是黄绿色。而反交时，坐果率明显降低，坐果率最高的组合为亚布力杜鹃x牛皮杜鹃。为36.151％：与迎红杜鹃的杂交坐果率为6．52％；而与大字杜鹃的杂交坐果率为O，但反交时亚布力×牛皮杜鹃的种胚萌发率极显著地高于正交。为39．81％，而且种胚蒴生长健壮，无需继代，可直接进行炼茁移裁。

关 键 词：Rh. chrysanthum Pall. Interspecific cross compatibility Embryo culture 

分 类 号：S685.21[农业科学—观赏园艺]