Fatty Acid Composition and Seed Quality Traits of the Transgenic Rapeseed W-4(Brassica napus L.) with Down-regulated Expression of fad2 Gene  

fad2基因下调表达的甘蓝型油菜W-4株系脂肪酸及品质分析（英文）

作　　者：陈松[1] 彭琦[1] 高建芹[1] 周晓婴[1] 张洁夫[1] 浦惠明[1] 戚存扣[1] 

机构地区：[1]江苏省农业科学院经济作物研究所/国家油菜改良中心南京分中心/农业部长江下游棉花与油菜重点实验室,江苏南京210014

出　　处：《Agricultural Science & Technology》2015年第8期1769-1774,共6页农业科学与技术（英文版）

基　　金：Supported by National Rapeseed Industrial Technology System(CARS-13);"948"Project of Ministry of Agriculture(2011-G23)~~

摘　　要：To clarify the effect of down-regulated expression of fad2 gene on the seed nutritional quality of rapeseed, the fatty acid composition, amino acid composi- tion, oil content, protein content, crude fiber content and glucosinolate content in the seeds of both transgenic line W-4 and its control Westar were compared. The re- sults showed that the oleic acid content in W-4 was 86.03%±0.20%, which was 29.36% higher than that in the control （P〈0.01）; the linoleic acid content was 2.86%± 0.01%, which was reduced by 84.03% compared with that in the control （P〈0.01）; the linolenic acid content in W-4 was 3.04%±0.04%, reduced by 57.60% （P〈0.01）; the palmitic acid content in W-4 was 3.23%±0.07%, reduced by 18.63% （P〈0.01）; the eicosenoic acid content in W-4 was increased by 18.46% compared with that in the control （P〈0.01）; the erucic acid content in W-4 was increased by 13.15% （P〈 0.05）; there was no significant difference in stearic acid content between the treat- ment and control groups （P〉0.05）. The amino acid composition analysis showed that total 18 amino acids, including 8 essential amino acids, were detected in both W-4 and Westar; there were no significant differences in contents of the 18 amino acids between the treatment and control groups except that of tyrosine （P〉0.05）; the contents of oil, proteins, glucosinolates and crude fiber in W-4 were 45.40%± 0.17% （P〉0.05）, 18.18%±0.91% （P〉0.05）, 18.20%±1.21% （P〉0.05） and 12.29%± 0.04% （P〉0.05）, respectively. All the results above showed that the down-regulated expression of fad2 had great effects on fatty acid composition and accumulation in rapeseed seeds, but had no significant effects on other seed quality traits, such as oil content, protein content, crude fiber content and glucosinolate content.为研究甘蓝型油菜种子fad2基因下调表达对油菜籽营养品质的影响,分析比较了转基因高油酸油菜品系W-4和非转基因对照Westar种子中的脂肪酸、氨基酸组成,以及油份、蛋白质、纤维素和硫甙葡萄糖甙含量等品质指标。数据显示W-4种子中油酸含量达到86.03%±0.20%,较对照增加29.36%,达极显著水平;亚油酸和亚麻酸含量分别为2.86%±0.01%和3.04%±0.04%,较对照分别下降84.03%和57.60%,降幅达极显著水平;棕榈酸含量为3.23%±0.07%较对照Westar降低了18.63%,达极显著水平,而硬脂酸含量差异不显著;廿碳烯酸和芥酸分别较对照增加18.46%和13.15%,达极显著和显著水平;氨基酸组成分析结果显示,W-4和对照种子中均可检测到包括8种必需氨基酸在内的18种氨基酸,除酪氨酸含量低于对照外,其余的差异均不显著;而W-4含油量、蛋白质、硫甙葡萄糖甙和纤维素含量分别为45.40%±0.17%、18.18%±0.91%、18.20%±1.21%和12.29%±0.04%,与对照差异不显著;结果表明油菜种子中fad2基因下调表达对种子的脂肪酸合成与积累影响最大,而对种子油份含量、蛋白质含量、纤维素含量以及硫甙葡萄糖甙的含量无显著影响。

关 键 词：Transgenic rapeseed fad2 gene silencing Quality traits 

分 类 号：S565.4[农业科学—作物学]