Analysis on Bacterial Community Structure in Mushroom(Agaricus bisporus) Compost Using PCR-DGGE  

双孢蘑菇堆肥过程中细菌群落结构分析（英文）

作　　者：郭亚萍[1] 张国庆[2] 陈青君[1] 杨凯[1] 

机构地区：[1]北京农学院植物科学技术学院农业应用新技术北京市重点实验室,北京102206 [2]北京农学院生物科学与工程学院农业部都市农业(北方)重点实验室,北京102206

出　　处：《Agricultural Science & Technology》2015年第8期1778-1784,共7页农业科学与技术（英文版）

基　　金：Supported by the National Key Technology Research and Development Program during the 12th Five-Year Plan Period of China(2012BAD14B09);Earmarked Fund for China Agriculture Research System(PXM2013-014207-000096);Beijing Higher Education Young Elite Teacher Project(YETP1714)~~

摘　　要：This study aimed to investigate the bacterial communities in mushroom compost piles composed of rice straw, corn stover, and cow dung. Bacterial com- munities of samples at the beginning of composting, at the end of fermentation phase I and II were collected and analyzed using Polymerase Chain Reaction-De- naturing Gradient Gel Electrophoresis （PCR-DGGE） based on 16S rDNA universal primers from Escherichia coli. A total of 56 different clone sequences were obtained （GenBank accession number： KF630598-KF630653）. They were classified into seven phyla and 42 genera. Dominant microflora during composting belonged to phylum Proteobacteria, Firmicutes, and Actinobacteria, with the dominant genera of Bacillus, Paenibacillus, Thermomonospora, Thermasporomyces, Pseudomonas, and Cellvibrio. Bacterial diversity （Shannon index） analysis showed that bacterial species in com- post pile composed mainly of rice straw continuously increased during composting, while those in compost pile composed mainly of corn stover firstly increased and then reduced. Principal component analysis showed that corn stover compost sam- ples at the end of fermentation phase I and phase II were clustered into one group, suggesting that corn stover composted faster than anticipated. In general, rice straw compost has higher bacterial diversity but longer composting time period, while corn stover compost has lower bacterial diversity but shorter composting time period.分别以玉米秸、牛粪和稻草、牛粪为主要原料,设计两种双孢蘑菇(Agaricus bisporus)堆肥配方并进行堆肥发酵,研究二者堆肥过程中细菌群落多样性。在建堆、一次发酵结束和二次发酵结束3个时期分别采集堆肥样品,提取总DNA,以大肠杆菌16S r DNA序列设计通用引物,进行PCR-DGGE扩增和序列分析。结果获得56条特异条带16S r DNA基因信息(Gen Bank登录号为KF630598-KF630653),分属于细菌的7个门、42个属。两种配方堆肥过程中主要类群属于变形菌门(Proteobacteria)、厚壁菌门(Firmicutes)和放线菌门(Actinobacteria),主要的优势种属包括Bacillus属、Paenibacillus属、Thermomonospora属、Thermasporomyces属、Pseudomonas属和Cellvibrio属。多样性指数分析显示,在堆肥过程中,稻草配方微生物多样性变化呈连续上升趋势,而玉米秸配方堆肥微生物多样性呈先升高、再降低的趋势。主成分分析(PCA)显示,玉米秸配方一次发酵结束的样品与二次发酵结束的样品聚为一类,说明玉米秸配方堆肥比预期提前腐熟。本研究表明,稻草配方微生物多样性高,所需腐熟时间长;玉米秸配方微生物多样性略低,但所需腐熟时间大大缩短。

关 键 词：PCR-DGGE A qaricus bisporus COMPOST Bacterial community 

分 类 号：S141.4[农业科学—肥料学]