隔药饼灸对FGIDs肝郁脾虚模型大鼠胃肠组织中SP表达及尿液D-木糖排泄率的影响  

Influence of cakeseparated moxibustion on gastrointestinal expression of substance P and urinary excretion of D-xylose in functional dyspepsia rats with liver-stagnation and spleendeficiency syndrome

作　　者：葛君芸[1] 常小荣[1] 刘密[1] 刘未艾[2] 黄河[1] 呙安林 沈菁[1] 

机构地区：[1]湖南中医药大学针灸推拿学院,湖南省长沙市410007 [2]湖南中医药大学第二附属医院针灸科,湖南省长沙市410005

出　　处：《世界华人消化杂志》2015年第20期3265-3269,共5页World Chinese Journal of Digestology

基　　金：国家重点基础研究发展计划(973计划)基金资助项目;No.2009CB522904;国家自然科学基金资助项目;No.81173326;国家自然科学青年基金资助项目;Nos.81303049;81403486;湖南省教育厅科学研究基金资助项目;No.13C701~~

摘　　要：目的:观察隔药饼灸对功能性胃肠病(functional gastrointestinal disorders,FGIDs)肝郁脾虚模型大鼠胃肠组织中P物质(substance P,SP)表达及尿液D-木糖排泄率的影响,探讨隔药饼灸对FGIDs的治疗作用及其作用机制.方法:将36只大鼠随机分为空白组、模型组和隔药饼灸组.每组12只,除空白组外,其余2组采用复合病因造模法进行造模.3组大鼠于造模前与造模后都进行食量和体质量的测定,然后各组按被试因素施加方法,分别进行生理盐水和隔药饼灸14 d.治疗结束后,收集大鼠尿标本,用于检测大鼠尿D-木糖排泄率,再取胃和结肠组织,采用Western blot检测SP含量.结果:2组大鼠造模后,食量和体质量均较空白组大鼠明显减少(P<0.05),同时尿D-木糖排泄率较造模前明显下降(P<0.05);治疗后,C组尿D-木糖排泄率较治疗前明显上升(P<0.05);SP含量:与A组相比,B、C组大鼠胃窦和结肠部位SP含量显著升高(P<0.01);与B组相比,C组大鼠各组织中SP含量显著下降(P<0.01).结论:隔药饼灸通过调节SP表达,从而达到改善胃肠运动功能的作用,这可能是其作用机制之一.AIM： To observe the influence of cake-separated moxibustion on gastrointestinal expression of substance P （SP） and urinary excretion of D-xylose in functional dyspepsia rats with liver- stagnation and spleen-deficiency syndrome, and to explore the mechanisms underlying the therapeutic effect of cake-separated moxibustion on functional dyspepsia. METHODS： Thirty-six rats were randomly divided into a control group （A）, a model group （B） and a cake-separated moxibustion group （C）, with 12 rats in each group. Except the control group, the other two groups were subjected to induction of functional dyspepsia using a composite modeling method. Food intake and body weight were measured before and after modeling. Saline and cake-separated moxibustion were applied to the corresponding groups for 14 d. After treatment, urine sample were collected for the detection of urinary D-xylose excretion rate, and stomach and colon tissues were collected to determine the expression of SP using Western blot. RESULTS： After modeling, food intake, body weight and urinary D-xylose excretionrate were significantly reduced in groups B and C compared with group A （P 〈 0.05）. After treatment, urinary D-xylose excretion significantly increased in group C compared with group B （P 〈 0.05）. Compared with group A, SP expression in the gastric antrum and colon was significantly increased in groups B and C （P 〈 0.01）. Compared with group B, SP expression in group C decreased significantly （p 〈0.01）. CONCLUSION： Cake-separated moxibustion may regulate the expression of SP and improve gastrointestinal motor function, thus achieving the purpose of treatment of functional dyspepsia with liver-stagnation and spleen- deficiency syndrome.

关 键 词：隔药饼灸 功能性消化不良 P物质 D-木糖 

分 类 号：R246.1[医药卫生—针灸推拿学]