丽水市HIV感染长期不进展者HLA-A、HLA-B和HLA-DRB1基因多态性的研究  

Study on polymorphisms of HLA-A,HLA-B and HLA-DRB1 loci among HIV long-term nonprogressors in Lishui

作　　者：叶灵[1] 雷永良[1] 陈沙彬[1] 叶碧峰[1] 陈秀英[1] 

机构地区：[1]丽水市疾病预防控制中心,浙江丽水323000

出　　处：《中国艾滋病性病》2015年第8期656-659,共4页Chinese Journal of Aids & STD

基　　金：浙江省医药卫生科技计划项目(2014KYB318);丽水市公益性技术应用项目(2013JYZB57)~~

摘　　要：目的从基因水平了解丽水市艾滋病病毒(HIV)感染长期不进展者(LTNPs)与典型进展者(TPs)的人类白细胞抗原(HLA)-A、HLA-B和HLA-DRB1位点等位基因频率,比较两类人群的差异,初步探索保护性基因和易感基因。方法应用聚合酶链反应-序列特异性引物技术(PCR-SSP),对丽水市42例LTNPs和48例TPs进行HLA-A、HLA-B和HLA-DRB1等位基因分型。结果共鉴定出9个HLA-A等位基因,16个HLA-B等位基因,12个HLA-DRB1等位基因。HLA-A*02频率LTNPs组(44.05%)高于TPs组(20.83%)(P<0.01),而A*11频率则是LINPs组(20.24%)低于TPs组(42.71%)(P<0.01);HLA-B*40频率LTNPs组(17.86%)低于TPs组(28.13%)(P>0.05);HLA-DRB*15频率LTNPs组(5.95%)低于TPs组(29.17%)(P<0.01);LTNPs组HLA基因座纯合子频率(15.87%)低于TPs组(33.33%)(P<0.01)。结论在该人群中,HLA-A*02等位基因可能与延缓艾滋病疾病进程相关,而HLA-A*11、B*40、DRB1*15可能加速艾滋病疾病进程。含HLA基因杂合子者对疾病显示出更好的抵御能力。Objective To explore allele frequency of HLA-A,HLA-B,HLA-DRB1 among HIV-infected longterm nonprogressors（LTNPs）and Typical progressors（TPs）in Lishui and to compare the difference between the two groups as well as to analyze the susceptible and protective genes.Method Polymerase chain reaction-sequence primers（PCR-SSP）were used to determine HLA-A,HLA-B,HLA-DRB1 alleles among 42 LTNPs and 48 TPs in Lishui.Results 9A,16 Band 12DRB1alleles were detected.The allele frequency of HLA-A＊02in LTNPs was higher than that in TPs（44.05%,20.83%,P〈0.01）,and A＊11frequency was lower than that in TPs（20.24%,42.71%,P〈0.01）.HLA-B＊40allele frequency in LTNPs was lower than that in TPs（17.86%,28.13%,P〈0.05）;HLA-DRB1＊15frequency in LTNPs was also lower than that in TPs（5.95%,29.17%,P〈0.01）.The frequency of homozygous HLA loci in LTNPs was lower than in TPs.Conclusion HLA-A＊02alleles may be association with slower AIDS progression,whereas HLA-A＊11,HLA-B＊40and HLA-DRB1＊15alleles are potentially association with faster disease progression.Those who have homozygous HLA loci would promote the progress of the disease.

关 键 词：艾滋病病毒 长期不进展者 等位基因 

分 类 号：R373.9[医药卫生—病原生物学]