复方痛风康对高尿酸血症模型大鼠肾功能的保护作用及影响IL-1β、TNF-α、TGF-β_1的研究  

Study on Chinese Herbal Compound Tongfengkang in Protecting Renal Function and Effecting IL-1β,TNF-α,TGF-β_1 in Hyperuricemia Rats

作　　者：郑媛[1] 王淳[1] 李伟[1] 杨旭[1] 刘畅[1] 张建军[1] 

机构地区：[1]北京中医药大学,北京100029

出　　处：《中国中西医结合肾病杂志》2015年第7期575-578,I0001,共5页Chinese Journal of Integrated Traditional and Western Nephrology

基　　金：国家自然科学基金资助项目(No.81273632)

摘　　要：目的:探讨以清热利湿化瘀散结法创制的中药复方痛风康对高尿酸血症模型大鼠血尿酸、肾功能、肾组织病理改变的影响,以及对肾脏组织中白细胞介素1(IL-1β)、肿瘤坏死因子-α(TNF-α)、转化生长因子-β1(TGF-β1)表达的影响。方法:将雄性SD大鼠随机分为空白组、模型组、苯溴马隆组及复方痛风康高、中、低剂量组,共6组。除空白组外,其余各组均以腺嘌呤和酵母膏灌胃,同时各给药组灌胃给予相应的药物,空白组和模型组给予等体积蒸馏水,连续21 d。末次给药后,测定大鼠血清尿酸(UA)、尿素氮(BUN)、肌酐(Scr)水平;放射免疫法测定大鼠肾脏组织IL-1β、TNF-α、TGF-β1的含量;制作肾组织病理切片,光镜下观察肾组织超微病理学的改变。结果:模型组动物血清UA、BUN、Scr水平升高,与空白组、苯溴马隆组比较,差异有统计学意义(P<0.05)。痛风康高剂量组UA含量明显降低,与空白组比较,差异有统计学意义(P<0.01)。用药各组与模型组相比BUN、Scr含量均明显降低,差异有统计学意义(P<0.05)。模型组肾组织IL-1β、TNF-α、TGF-β1含量与空白组比较,显著升高(P<0.01)。苯溴马隆组、痛风康高、中、低剂量组大鼠肾组织IL-1β、TNF-α、TGF-β1含量较模型组均有所降低。其中痛风康高剂量组大鼠肾组织IL-1β、TNF-α含量与模型组相比,差异有统计学意义(P<0.01,P<0.05)。肾脏病理变化以模型组损伤最为明显,各给药组均有不同程度的改善。结论:以清热利湿化瘀散结法创制的中药复方痛风康,在降低高尿酸血症大鼠血清尿酸的同时,可降低炎性因子含量,促使抗炎症因子的产生,从而减轻肾组织炎性反应,对肾脏功能和形态的损伤具有一定的保护作用。Objective：To evaluate the effects of Chinese herbal compound Tongfengkang（TFK） on uric acid（UA）, renal function, pathological impairment of kidney, as well as the interleukin - 1β （ IL - 1β）, tumor necrosis factor - a（ TNF - α） and trans- forming growth factor - β1 （ TGF - β1 ） in the rats with hyperuricemia. The Chinese herbal compound TFK is based on the traditional therapeutics of cleating away heat and dampness（清热利湿） and promoting circulation of blood and removing stasis（ 活血散结）. Methods： Male SD rats were randomly divided into normal group, model group, Benzbromarone group, high, medium and low - dose Chinese herbal compounds Prescription（ HC,MC and LC） groups in three doses. In addition to the normal group, the rest ones were offered with Adenine and Yeast extracts for 21 days. While the rats of the administration group had been administered appropriate drugs, the normal group and the model group were given by gavage the distilled water for 21 days. Then blood was collected, the ser- um levels of UA, blood urea nitrogen（BUN） and serum creatinine（Scr）were measured; the IL- 1β, TNF- α and TGF- β1 content in serum were detected by radiommunoassay. The pathologic changes in kidney were observed by electronmicroscope. Results：The levels of UA, BUN, Scr in the model group Compared to the normal group was significantly increased（P 〈 0.05 ）. The level of UA in the High group Compared to the normal group was significantly decreased（P 〈0.01 ）. Compared with the model group. The levels of BUN, Scr in each drug groups evidently decreased of treated rats （P 〈 0.05 ）, the concentrations of IL - 1β, TNF - β and TGF - β1 were evidently decreased in serumin. The concentrations of IL - 1β, TNF - a and TGF - β1 in the model group Compared to the nor- mal group were significantly increased（ P 〈0.01 ）. The eoncentrations of IL- 1β, TNF- α in the High group Compared to the model group were significantly difference （P 〈 0. 01,

关 键 词：高尿酸血症 IL-1Β TNF-α TGF-β1肾功能 病理学 

分 类 号：R285.5[医药卫生—中药学] R-332[医药卫生—中医学]