一种高哌嗪溴盐的液相分析新方法  

A new method for liquid phase analysis of homopiperazine bromine salt

作　　者：魏凤[1] 岳涛[1] 孟宪兴 周倜[1] 刘茂玲[1] 冯维春[1] 

机构地区：[1]山东省化工研究院山东省生物化学工程重点实验室,山东济南250014

出　　处：《现代化工》2015年第8期185-186,188,共3页Modern Chemical Industry

基　　金：山东省政府"泰山学者"建设工程资金项目(ts20130918)

摘　　要：建立了一种高效液相色谱法测定高哌嗪溴盐的新方法,确定了最优的色谱条件,色谱柱采用岛津VP-ODS色谱柱,柱长为250 mm,内径为4.6 mm;流动性采用V(甲醇)∶V(水)=10∶90(水相含30 mmol/L磷酸二氢钾,稀酸调p H=3.5);检测波长为220 nm;流速为1.0 m L/min;进样量为2.0μL。该分析方法填补了国内空白,分离度R≥1.5,线性相关系数达到了0.999以上,标准偏差均小于1.4%,加标回收率在98%~103%之间。实验结果表明,该方法能够满足工艺中控分析的需要。A new method for determination of high piperazine bromine salt based on high performance liquid chromatography （HPLC） is developed. The optimal operation parameters are shown as follows ： VP-ODS chromatographic column ,250 mm of column length with 4. 6 mm of inner diameter, mixture of methanol and water （90/10） （water phase containing 30 mmol/L of potassium dihydrogen phosphate, pH = 3.5 ） as mobile phase ,220 nm of detection wavelength, 1.0 mL/min of the flow rate and 2. 0 p^L of sample. The separation degree of this method is no less than 1.5. The linear correlation coefficient is above 0. 999 with the standard deviation less than 1.4%. The recovery of standard addition is between 98% and 103%. It indicates that the new method can meet the needs of the centurial control analysis.

关 键 词：高哌嗪溴盐 高哌嗪 液相色谱 

分 类 号：O657[理学—分析化学]