去白细胞悬浮红细胞储血袋两种放置方式的溶血性分析  

Hemolytic Analysis of Two Kinds of Placement of Leukoreduced Suspension Red Blood Cells Storage Bag

作　　者：张瑞君[1] 段秉政[3] 鞠春梅[1] 宋艳红[2] 白燕[2] 霍蓉[2] 隋素琴[1] 

机构地区：[1]内蒙古医科大学第四附属医院输血科,内蒙古包头014030 [2]内蒙古包头市中心医院输血科,内蒙古包头014040 [3]内蒙古医科大学第四附属医院内科,内蒙古包头014030

出　　处：《现代检验医学杂志》2015年第4期120-122,共3页Journal of Modern Laboratory Medicine

基　　金：内蒙古包头市卫生基金资助项目（wsjj2012067）.

摘　　要：目的：探讨去白细胞悬浮红细胞储血袋两种不同放置方式对上清液游离血红蛋白（FHb）和红细胞溶血率的影响，保证临床输血的安全、有效。方法选择20例无偿献血者每人400 ml 的全血，24 ml 内制成去白细胞悬浮红细胞制品，将其等量均匀分装成10袋，随机分成2组，每组5袋，一组以直立放置，一组以水平放置，在同一环境条件下储存，分别在储存的第7天，14，21，28，35天时随机从各组取出1袋血液，分别测定其 FHb 和红细胞溶血率，进行统计学分析。结果FHb 和红细胞溶血率在贮存的第21天时检测的结果，直立放置组分别为（217.310±48.477）mg／L 和（0.250±0.056）％，水平放置组分别为（173.972±39.027）mg／L 和（0.189±0.045）％，直立放置组的结果要高于水平放置组，统计学结果分别为 t＝3.114，P ＝0.003＜0.05和 t＝3.798，P ＝0.001＜0.05，差异有统计学意义。结论在血液储存期内，储血袋水平方式放置可减少红细胞破坏，对血液的贮存更加有利。Objective Discussion leukoreduction of red blood cells suspended in two different storage bag placement and he-molysis rate impact on the supernatant free hemoglobin （FHb）,to ensure the clinical transfusion is safe and effective.Meth-ods Selected 20 donors to sample 400 ml whole blood per person to make leukodepleted red blood cells,which were evenly divided into 10 bags.The 10 bags were randomly divided into two groups,one to the upright position,one group of horizon-tal.The two groups were stored under the same conditions.Respectively,in the 7,14,21,28,35 day,randomly removed one storage bag from each group,FHb and red blood cell hemolysis rate were measured and analyzed statistically.Results FHb and hemolysis rate results stored in the first 21 days of testing,uprightgroup were （217.310±48.477）mg/L and （0.250± 0.056）%,respectively horizontal group （173.972±39.027）mg/L and （0.189±0.045）%,the results set upright than hori-zontal group,the results were statistically（t=3.114,P =0.003〈0.05 and t=3.798,P =0.001〈0.05）,the difference was statistically significant.Conclusion In the blood storage period,storage bags can be placed horizontally to reduce the de-struction of red blood cells,blood storage is more favorable.

关 键 词：储血袋 贮存时间 放置方式 游离血红蛋白 溶血率 

分 类 号：R457.12[医药卫生—治疗学]