Beckman Coulter 实验室自动化系统生化免疫样品间携带污染评估与解除  

Evaluation and Disposal of the Carryover between the Chemistry and Immunoassay in Beckman Coulter Laboratory Automation System

作　　者：夏勇[1] 纪玲[1] 徐安平[1] 林松[1] 薛灏[1] 郑若洋[1] 

机构地区：[1]北京大学深圳医院检验科,广东深圳518036

出　　处：《现代检验医学杂志》2015年第4期125-127,共3页Journal of Modern Laboratory Medicine

基　　金：北京大学深圳医院学科骨干科研基金启动项目（项目编号：201412）.

摘　　要：目的：评估 Beckman Coulter 实验室自动化系统生化免疫样品间携带污染情况，探讨不选择分杯模块时生化免疫共用一管血的可行性。方法将高浓度及不同样品量（2000μl，500μl）低浓度的 HCG 样品按照一定顺序在 Beckman AU5421全自动生化分析仪上检测生化项目，比较检测生化项目前后低浓度样品 HCG 含量的变化评估生化免疫样品间携带污染情况，并在增加 Beckman AU5421全自动生化分析仪特殊清洗程序后重复试验。结果在不同样品量（2000μl，500μl）条件下，Beckman AU5421全自动生化分析仪单模块及整机携带污染分别为5.44，15.47，23.51，45.96 ppm，不同样品量及单模块与整机间携带污染差异均有统计学意义（t ＝14.553，P ＜0.001；t ＝5.527，P ＝0.005；t ＝3.985，P ＝0.016；t＝20.457，P ＜0.001）；增加 Beckman AU5421特殊清洗程序后仅检出500μl 样品量时整机携带污染为0.22 ppm。结论不选择分杯模块时，Beckman Coulter 实验室自动化系统可使用特殊清洗程序解决生化免疫间携带污染。Objective To evaluate the Carryover between the chemistry and immunoassay in Beckman Coulter Laboratory Au-tomation System and decide to whether sharing samples for testing between chemistry and immunoassay systems or not. Methods According to a certain order,high concentration samples and low concentration samples of HCG with different sample volume （500 μl,2 000 μl）were tested on Beckman AU5421 automatic biochemical analyzer.The HCG of low concen-tration samples were then tested to evaluate the carryover between the chemistry and immunoassay and explored the correc-tive procedure to deal with the carryover by increasing special cleaning process of beckman AU5421 automatic biochemistry analyzer.Results Under different sample volume,the carryover in a single module and as a whole of the beckman AU5421 automatic biochemistry analyzer were 5.44,15.47,23.51 and 45.96 ppm respectively （t=14.553,P 〈0.001;t=5.527,P =0.005;t=3.985,P =0.016;t=20.457,P 〈0.001）.By increasing special cleaning process the carryover of 0.22 ppm was detected in 500 μl sample volume of the beckman AU5421 automatic biochemistry analyzer as a whole.Conclusion The car-ryover between the chemistry and immunoassay in Beckman Coulter Laboratory Automation System could been sovled by in-creasing special cleaning process of beckman AU5421 automatic biochemistry analyzer.

关 键 词：实验室自动化系统 样品间 携带污染 

分 类 号：R446.112[医药卫生—诊断学]