直接抗人球蛋白试验阳性患者交叉配血方法探讨  

Exploration of Appropriate Cross-Matching Methods for Positive Patients of the Direct Antiglobulin Test

作　　者：李建刚[1] 赵家宁[1] 徐华[2] 姚锁良[3] 

机构地区：[1]西安市第四医院输血科,西安710004 [2]陕西省血液中心,西安710061 [3]西安交通大学医学院附属红会医院输血科,西安710054

出　　处：《现代检验医学杂志》2015年第4期130-132,共3页Journal of Modern Laboratory Medicine

摘　　要：目的：探讨直接抗人球蛋白试验（DAT）阳性患者的交叉配血方法。方法采用 DAT 法筛查红细胞表面的不规则抗体，微柱凝胶法进行血型鉴定，凝聚胺法和抗人球蛋白法交叉配血。结果共计5例 DAT 阳性的疑难配血患者，其血型分别为 A-DCcEe，O-DCCee，O-DCcee，A-DCCee 和 A-DCcee。选择相应血型抗原阴性的供者血样进行交叉配血，凝集强度均弱于自身对照，建议观察输注洗涤红细胞，患者未发生不良反应。结论对于 DAT 阳性的患者，可配合性输注相应血型抗原阴性的洗涤红细胞。Objective To investigate the appropriate cross-matching methods for the direct antiglobulin test （DAT）positive patients.Methods The DAT was used to screen the irregular antibodies on the surface of erythrocytes.The microcolumn gel test was used for blood identification.The antiglobulin test and manual polybrene test were used for cross-matching.Re-sults A total of five DAT positive cases were collected in study.The blood groups of each case were detected,including A-DCcEe,O-DCCee,O-DCcee,A-DCCee and A-DCcee.The donor samples with corresponding blood-antigen negative were cho-sen for cross-matching,and the agglutination intensity of each cross-matching tube was weaker than the control.The transfu-sion of washed red blood cells was suggested.Finally,each transfusion was safe and efficient.Conclusion The infusion of the washed red blood cells with corresponding blood-antigen negative could efficiently ensure the safety of blood transfusion.

关 键 词：直接抗人球蛋白试验 交叉配血 洗涤红细胞 

分 类 号：R457.12[医药卫生—治疗学]