疫苗生产中使用的酸性和碱性苯酚对MRC-5细胞生长的影响  

Effect of acid phenol and alkaline phenol used in vaccine production on growth of MRC-5 cells

作　　者：杨月莲[1] 王亮[1] 周锋[1] 谢蕾[1] 沈谊清[2] 蒋樱[1] 王伟[1] 周翎乔[1] 

机构地区：[1]上海生物制品研究所有限责任公司生物技术制品中心,200051 [2]上海生物制品研究所有限责任公司生产部,200051

出　　处：《国际生物制品学杂志》2015年第4期166-168,共3页International Journal of Biologicals

摘　　要：目的：观察疫苗生产中使用的消毒剂酸性苯酚和碱性苯酚对M RC‐5细胞生长的影响。方法将酸性苯酚（0．37％）和碱性苯酚（0．74％）分别加入M RC‐5细胞培养瓶中，观察细胞生长形态，绘制细胞生长曲线，测定细胞贴壁率。分别采用 t检验和χ2检验对结果进行比较。结果加入酸性和碱性苯酚后，第7天的细胞数分别为6．65×10^5和7．12×10^5，与对照组（6．93×10^5）相比，差异无统计学意义（t＝0．476，P＝0．654；t＝0．334，P＝0．752）；第8小时的细胞贴壁率分别为88．3％和96．0％，与对照组（90．3％）相比，差异亦无统计学意义（χ^2＝0．765，P＝0．659；χ^2＝0．376，P＝0．585）。结论微量酸性和碱性苯酚对M RC‐5细胞的生长没有显著影响。Objective To investigate effect of acid phenol and alkaline phenol used in vaccine production as disinfectants on MRC‐5 cell growth .Methods Acid phenol（0 .37% ） and alkaline phenol （0 .74% ）were added to MRC‐5 cell culture ,respectively .The morphology and growth curve of cells were observed ,and cell attachment rate determined .The results were compared using t‐test and χ2‐test . Results The number of MRC‐5 cells added with acid phenol （6 .65 × 10^5 ） and alkaline phenol （7 .12 × 10^5 ） had no difference from that of control cells （6 .93 × 10^5 ） at 7 d （t= 0 .476 ,P= 0 .654 ;t=0 .334 ,P=0 .752） .No difference of attachment rate was observed between the treated cells （88 .3%and 96 .0% ） and control cells （90 .3% ） at 8 h （χ2 = 0 .765 ,P= 0 .659 ;χ2 = 0 .376 ,P= 0 .585 ） . Conclusion Trace amount of acid and alkaline phenols has no significant effect on growth of MRC‐5 cells .

关 键 词：细胞 消毒剂 酸性苯酚 碱性苯酚 

分 类 号：R852.22[医药卫生—航空、航天与航海医学]