Ethnopharmacological Investigation and Rapid Authentication of Mongolian Patent Medicines Digeda  

作　　者：Le Zhang Zhan-hu Cui Yong-xu Mu Kun-hua Wei Zhen-hua Li Hong Zhu Da-wei Yang Ying-li Wang Ping Long Chun-hong Zhang Min-hui Li 

机构地区：[1]Baotou Medical College [2]Guangxi Botanical Garden of Medicinal Plants [3]Nanyang Institute of Technology [4]The First Affiliated Hospital of Baotou Medical College [5]Inner Mongolia Institute of Traditional Chinese Medicine

出　　处：《Chinese Herbal Medicines》2015年第3期223-237,共15页中草药（英文版）

基　　金：National Natural Science Foundation of China(81160504,81060372);"Twelfth Five-year Plan"Program supported by the Ministry of Science and Technology(2012BAI28B02);Guangxi Natural Science Foundation of China(2011GXNSFD018037)

摘　　要：Objective To investigate Mongolian medicinal plants called Digeda and the prescriptions in Inner Mongolia region and to establish a molecular method for authentication of Digeda Mongolian patent medicines （MPMs）. Methods A field investigation was conducted on traditional uses of Digeda. After interviewed traditional healers in Mongolian, ethnopharmacological information of Digeda prescriptions was recorded in detail, including names, compositions, and traditional uses. And the total DNA from 10 MPMs has been amplified by three pairs of specific primers. Specific PCR products were further identified by sequence alignment with the known sequences already submitted in GenBank or own sequences. Results Fifteen Digeda plants and 29 Digeda prescriptions with their ethnopharmacological knowledge were collected. Ten MPM samples containing Lomatogonium rotatum, Viola philippica, and Corydalis bungeana were successfully evidenced by PCR with specific bands as raw materials. Conclusion Digeda should be further investigated in ethnopharmacology, which is a fundamental step toward developing efficacious natural drugs for various diseases. PCR amplification of specific allele is an easy and economical method, which can be used to identify highly processed MPMs and will assist in monitoring their qualities and legalities.Objective To investigate Mongolian medicinal plants called Digeda and the prescriptions in Inner Mongolia region and to establish a molecular method for authentication of Digeda Mongolian patent medicines （MPMs）. Methods A field investigation was conducted on traditional uses of Digeda. After interviewed traditional healers in Mongolian, ethnopharmacological information of Digeda prescriptions was recorded in detail, including names, compositions, and traditional uses. And the total DNA from 10 MPMs has been amplified by three pairs of specific primers. Specific PCR products were further identified by sequence alignment with the known sequences already submitted in GenBank or own sequences. Results Fifteen Digeda plants and 29 Digeda prescriptions with their ethnopharmacological knowledge were collected. Ten MPM samples containing Lomatogonium rotatum, Viola philippica, and Corydalis bungeana were successfully evidenced by PCR with specific bands as raw materials. Conclusion Digeda should be further investigated in ethnopharmacology, which is a fundamental step toward developing efficacious natural drugs for various diseases. PCR amplification of specific allele is an easy and economical method, which can be used to identify highly processed MPMs and will assist in monitoring their qualities and legalities.

关 键 词：Digeda molecular identification Mongolian patent medicinesl PCR amplification ofspecific allele 

分 类 号：R29[医药卫生—民族医学]