Oleuropein Improves Long Term Potentiation at Perforant Path-dentate Gyrus Synapses in vivo  

作　　者：Fei-fei Pu Song Yin Hong-ying Chen Zhi Dai Tian-xiu Qian Cheng-cheng Wang Hui Xu Xiao-ying Wang 

机构地区：[1]Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College [2]Department of Pharmacy, Maternal and Child Hospital of Xiangtan [3]China Resources Sanjiu Medical & Pharmaceutical Co.,Ltd.

出　　处：《Chinese Herbal Medicines》2015年第3期255-260,共6页中草药（英文版）

基　　金：Ministry of Science and Technology(No.2013ZX09201022);National Natural Science Foundation of China(No.81073137,81373418)

摘　　要：Objective To investigate the effect of oleuropein （OE） on long term potentiation （LTP） at hippocampal perforant path-dentate gyrus synapses in vivo. Methods An outer guide cannula, a monopolar recording electrode, and a bipolar stimulating electrode were implanted in the skull and extracellular recording technique was used to record the population spike in the dentate gyrus of anesthetized rats. Results Oleuropein significantly increased the basal synaptic transmission and the amplitude of population spike was increased from （11 7.6 ＋ 2.3）% to （134.9 _＋ 3.7）% after administration with OE. OE also accelerated LTP induction and maintenance, the population spike amplitude after high frequency stimulation was increased from （167.2 ± 12.8）% to （225.5 ± 15.5）% and the maintenance phase of LTP was from （182.1 ± 15.1）% to （210.5 ± 9.0）% respectively after administration with OE. Conclusion Present study showed that OE significantly improved different stages of LTP, which could be the molecular mechanism of its efficacy on attenuating AD-like pathology and delaying cognitive decline. OE can be a promising drug for AD and dementia.Objective To investigate the effect of oleuropein （OE） on long term potentiation （LTP） at hippocampal perforant path-dentate gyrus synapses in vivo. Methods An outer guide cannula, a monopolar recording electrode, and a bipolar stimulating electrode were implanted in the skull and extracellular recording technique was used to record the population spike in the dentate gyrus of anesthetized rats. Results Oleuropein significantly increased the basal synaptic transmission and the amplitude of population spike was increased from （11 7.6 ＋ 2.3）% to （134.9 _＋ 3.7）% after administration with OE. OE also accelerated LTP induction and maintenance, the population spike amplitude after high frequency stimulation was increased from （167.2 ± 12.8）% to （225.5 ± 15.5）% and the maintenance phase of LTP was from （182.1 ± 15.1）% to （210.5 ± 9.0）% respectively after administration with OE. Conclusion Present study showed that OE significantly improved different stages of LTP, which could be the molecular mechanism of its efficacy on attenuating AD-like pathology and delaying cognitive decline. OE can be a promising drug for AD and dementia.

关 键 词：Alzheimer＇s disease dentate gyrus long term potentiation OLEUROPEIN population spike 

分 类 号：R285[医药卫生—中药学]