实时荧光PCR技术检测HLA-B27基因的临床应用研究  

Clinical application of real-time PCR technology in the HLA-B27 gene detection

作　　者：马志超[1] 黄燕华 陈鑫苹[1] 徐卫华[1] 符生苗[1] 

机构地区：[1]海南省人民医院医学检验中心,海口570311 [2]琼海市人民医院病理科,海南琼海571400

出　　处：《中国优生与遗传杂志》2015年第8期8-9,36,共3页Chinese Journal of Birth Health & Heredity

摘　　要：目的探讨实时荧光PCR技术检测HLA-B27基因对于诊断强直性脊柱炎的临床应用价值。方法用实时荧光PCR技术对强直性脊柱炎疑似患者300例进行HLA-B27基因检测,并对阳性患者是否确诊为强直性脊柱炎进行追踪,统计其确诊率。结果 300例强直性脊柱炎疑似患者中,阳性135例,阳性率为45%,其中126例阳性患者确诊为强直性脊柱炎,确诊率为93.3%。结论实时荧光PCR技术应用于HLA-B27基因的检测具有较高的特异性和灵敏度,且操作简便、快速,对强直性脊柱炎的早期诊断和辅助诊断具有重要的临床应用价值。Objective：To investigate the clinical significant of real-time fluorescent PCR detecting on HLA-B27 gene in the detection ankylosing spondylitis（AS）.Methods：The HLA-B27 gene was detected in 300 cases of ankylosing spondylitis patients suspected with the real-time fluorescent PCR technique,the positive patients diagnosed as AS were traced,and the results were statistically analyzed.Results：300 cases of ankylosing spondylitis patients with suspected,135 cases were positive,the positive rate was 45%,of which 126 cases were diagnosed as ankylosing spondylitis positive patients,the diagnosis rate was 93.3%.Conclusion：The application of real time fluorescence PCR in detection of HLA-B27 gene has clinical significance for diagnosis of AS,and has good sensitivity and specificity.

关 键 词：HLA-B27基因 强直性脊柱炎 实时荧光PCR技术 

分 类 号：R440[医药卫生—诊断学] R593.23[医药卫生—临床医学]