屋尘螨变应原Derp1对人肥大细胞HMC-1释放类胰蛋白酶的影响  

Effect of house duct mite Derp1 on the secretion of tryptase from human mast cell line HMC-1

作　　者：唐慧[1] 王朵勤[1] 沈燕芸[1] 徐金华[1] 

机构地区：[1]复旦大学附属华山医院,上海200040

出　　处：《中国麻风皮肤病杂志》2015年第8期451-454,共4页China Journal of Leprosy and Skin Diseases

基　　金：国家自然科学基金青年项目(编号:81402603);上海市卫生局青年科研项目(编号:20124y032)

摘　　要：目的:明确屋尘螨变应原Derp1对人肥大细胞系HMC-1释放类胰蛋白酶的影响。方法:培养后的HMC-1细胞分为单纯培养液组、5μg/mL Derp1组和5μg/mL Derp1+100μmol/L PAR2拮抗剂(FSLLY)组,用ELISA方法检测各组类胰蛋白酶水平,显微镜观察HMC-1细胞脱颗粒率。结果:培养液组,Derp1组和Derp1+FSLLRY组中类胰蛋白酶水平分别为31.12±3.60 ng/mL、70.40±1.55 ng/mL和56.72±2.66 ng/mL。Derp1组和Derp1+FSLLRY组肥大细胞脱颗粒率分别为66.67%和16.67%。结论:屋尘螨变应原Derp1可以通过激活人肥大细胞HMC-1表面PAR2受体引起肥大细胞脱颗粒释放类胰蛋白酶。Objective： To determine the effect of house dust mite Derpl on the secretion of typtase from human mast eel1 line HMC-1. Methods： After cultured HMC-1 cells were incubated respectively with Derpl 5 μg,/mL and Derpl 5 μg/mL＋PAR2 antagonist （FSLLY） 100 μmol/L. The level of typtase from HMC-1 cells was detected by ELISA. The degranulation of HMC-1 cells was observed under microscope. Results： The levels of the typtase from HMC-1 cells in culture medium group, Derpl group and Derpl combined FSL- LY group were 31.12±3.60 ng/mL, 70.40±1.55 ng/mL and 56.72±2.66 ng/mL, respectively. The dcgranula- tion rates in Derpl group and Derpl combined FSLLY group were 66.67% and 16.67%. Conclusion： The PAR2 receptor can be activated by house dust mite Derpl, which results in typtase secretion of the human mast cell line HMC-1.

关 键 词：屋尘螨 肥大细胞 蛋白酶激活受体2 类胰蛋白酶 

分 类 号：R758.2[医药卫生—皮肤病学与性病学]