HIV-1潜伏感染体外实验模型研究进展  

Research Progress on in vitro Models of HIV-1 Latency

作　　者：康学军[1] 杨怡姝[1] 

机构地区：[1]北京工业大学生命科学与生物工程学院,北京100124

出　　处：《中国生物工程杂志》2015年第8期96-102,共7页China Biotechnology

基　　金：国家科技重大专项项目(2014ZX10005-002);抗病毒药物北京市国际科技合作基地;"2011计划"生物治疗协同创新中心资助项目

摘　　要：潜伏感染的静息记忆CD4+T细胞是清除HIV-1病毒的一个重要障碍。处于潜伏状态的病毒多以原病毒c DNA的形式整合至宿主基因组中,但是病毒基因表达处于沉默状态,因此潜伏感染的细胞难以受到病毒的致细胞病变效应或机体特异性细胞毒性T细胞的杀伤,也不易受到抗反转录病毒治疗药物的作用。如何减少潜伏感染的细胞储存库是艾滋病治疗中亟需解决的一个问题。体内及体外HIV-1潜伏感染模型有助于深入了解HIV-1潜伏感染的建立、维持或打破机制,评价潜伏感染再激活剂的活性。在此侧重于介绍采用永生化细胞系、原代静息CD4^+T细胞或活化的CD4+T细胞建立的HIV-1潜伏感染体外实验模型。Latent infection in resting memory CD^4＋T cells is the major barrier to HIV-1 eradication. Under most circumstances,proviral c DNA was integrated into the host genome. Due to the factors from virus or the host,the transcription was interrupted,without producing any detected viral proteins. So the latently infected cells could not suffer the cytopathic effect from the virus,the attack from cytotoxic T lymphocyte,or the antiviral effect from the anti-retroviral therapy. It is urgent to decrease the latent reservoir in AIDS treatment. In vivo or in vitro latency models are beneficial for further studying the mechanism of latency establishment,maintenance or reactivation. The focuses are mainly on the in vitro HIV-1 latency models using immortalized cell lines,primary resting CD^4＋T cells or activated CD^4＋T cells.

关 键 词：人免疫缺陷病毒1型 潜伏感染 再激活 体外实验模型 

分 类 号：Q819[生物学—生物工程]