叶下珠和苦味叶下珠指纹图谱的建立及比较  

Study and Comparison on HPLC Fingerprint of Phyllanthus Urinaria Linn and Phyllanthus Amarus Linn

作　　者：张鑫瑶[1,2] 许利嘉[1,2] 张伟[3] 

机构地区：[1]中国医学科学院北京协和医学院药用植物研究所,北京100193 [2]国家教育部中草药物质基础与资源利用重点实验室,北京100193 [3]澳门科技大学中药质量研究国家重点实验室,澳门999078

出　　处：《世界科学技术-中医药现代化》2015年第5期990-993,共4页Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology

基　　金：澳门科学技术发展基金(091/2012/A3):叶下珠联用核苷类药物减毒增效作用机制与药效物质基础研究;负责人:张伟

摘　　要：目的:建立叶下珠和苦味叶下珠指纹图谱,为其是否可以混用或代替使用提出物质基础的依据。方法:采用高效液相色谱法(HPLC),色谱柱为INDUSTRIES Epic C18 120A(5μm,250 mm×4.6 mm);流动相为乙腈(A)-水(0.1%磷酸,V/V)二元梯度洗脱;检测波长为254 nm;流速为1 m L·min-1;柱温为30℃;进样量为10μL。结果:建立了叶下珠和苦味叶下珠指纹图谱。结论:该方法简便、准确、重现性好,为其混用或代替使用提出物质基础的依据。This study was aimed to establish the HPLC fingerprint of Phyllanthus urinaria Linn and Phyllanthusamarus Linn, in order to provide evidences for the study on material basis. Analysis was performed on an INDUSTRIES Epic C18 120A（5 μm, 250 mm × 4.6 mm） column eluted with the acetonitrile（A）- water（0.1% phosphoric acid, V/V） gradient system as mobile phase. The wavelength was 254 nm and the flow rate was 1mL·min^-1. The column temperature was 30℃. The injection volume was 10 μL. The results showed that the HPLC fingerprint of Phyllanthus urinaria L. and Phyllanthus amarus L. were established. It was concluded that the method was simple, accurate and reproducible. This study provids experimental data for rapid quality identification and comprehensive evaluation of Phyllanthus urinaria L. and Phyllanthus amarus L..

关 键 词：叶下珠 苦味叶下珠 指纹图谱 物质基础研究 

分 类 号：R282[医药卫生—中药学]