高效液相色谱指纹图谱评价丹参胶囊脂溶性成分质量  

Quality Evaluation on Liposoluble Components in Danshen Capsule with HPLC Fingerprint

作　　者：张珺[1] 程月发[1] 郭兰[1] 刘颖硕[1] 

机构地区：[1]华北理工大学冀唐学院,唐山063300

出　　处：《世界科学技术-中医药现代化》2015年第5期994-999,共6页Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology

基　　金：唐山市科技局唐山市科学技术研究与发展计划基金项目(13130298z):丹参胶囊高效液相色谱指纹图谱研究;负责人:张珺

摘　　要：目的:建立丹参胶囊脂溶性成分的高效液相色谱指纹图谱,用来全面评价丹参胶囊脂溶性成分的质量。方法:采用高效液相色谱法,以Welch ultimate XB-C18(250 mm×4.6 mm,5μm)为色谱柱,乙腈-水为流动相进行梯度洗脱。柱温为25℃,流速为1 m L·min-1,检测波长为270 nm,进样量为10μL。结果:分别确立了两个厂家丹参胶囊脂溶性成分指纹图谱中的7个和11个共有峰,20批样品的相似度均在0.9以上。结论:该分析方法稳定性和重现性较好,可作为评价丹参胶囊脂溶性成分质量控制的依据。This study was aimed to establish the HPLC fingerprint of liposoluble components in Danshen capsule for quality evaluation. HPLC was run on a Welch ultimate XB-C18 column（250 mm × 4.6 mm, 5 μm） with acetonitrilewater as mobile phase in gradient elution at a flow rate of 1 mL·min^-1. The column temperature was 25℃. The detection wavelength was 270 nm. The injection volume was 10 μL. The results showed that 7 and 11 common peaks were selected as the HPLC fingerprint of liposoluble components in Danshen capsule from two manufactures, respectively. The similarities of 20 batches of samples were above 0.9. It was concluded that the analysis was stable and reproducible, which can be used as a basis for evaluating the quality control of liposoluble components in Danshen capsule.

关 键 词：丹参胶囊 脂溶性成分 高效液相色谱指纹图谱 质量控制 

分 类 号：R284.1[医药卫生—中药学]