不同产地蒙古黄芪中黄芪甲苷、黄芪皂苷Ⅰ、黄芪皂苷Ⅱ和黄芪皂苷Ⅲ含量的比较与分析  

Comparative Study on Determination of Astragaloside, Astragaloside Ⅰ, Astragaloside Ⅱ and Astragaloside Ⅲ in Mongolia Radix Astragali from Various Habitats

作　　者：史静超[1] 李浩铮 王永辉[1] 周然[1] 刘必旺[1] 

机构地区：[1]山西中医学院,太原030024

出　　处：《世界科学技术-中医药现代化》2015年第5期1016-1021,共6页Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology

基　　金：山西省科技厅科技攻关项目(20120313015):山西特色药材规范化种植技术研究--黄芪种子与黄芪药材的主要有效成分与药理作用比较研究及相关产品研制开发;负责人:王永辉;科学技术部"十二五"国家科技支撑计划项目(2011BAI07B01):华北地区黄芪规范化种植基地优化升级及系列产品综合开发研究;负责人:周然

摘　　要：目的:采用HPLC-ELSD法测定了16批不同产地蒙古黄芪中黄芪甲苷、黄芪皂苷Ⅰ、黄芪皂苷Ⅱ、黄芪皂苷Ⅲ的含量。方法:使用DIKMA Diamonsil C18(150 mm×4.6 mm,5μm)色谱柱,乙腈-水为流动相,梯度洗脱,流速:1 m L·min-1,柱温:30℃。ELSD检测器参数:漂移管温度90℃,空气流量2.8 L·min-1。采用SPSS 16.0进行聚类分析。结果:黄芪甲苷、黄芪皂苷Ⅰ、黄芪皂苷Ⅱ和黄芪皂苷Ⅲ在进样量分别为0.093 2-1.02μg(r=0.999 5),0.789-8.78μg(r=0.999 7),0.506-3.13μg(r=0.999 6),0.016 1-1.38μg(r=0.999 2)范围内,平均回收率分别为97.55%、98.61%、99.68%、98.58%,RSD分别为1.2%、1.3%、1.3%、1.2%。聚类分析结果表明,单一采用黄芪甲苷为指标的聚类分析无法区分各省份蒙古黄芪,而同时采用4种皂苷含量为指标,可将不同省份蒙古黄芪明显区分开。结论:本方法简单、快捷、准确,能够更加直接地反映出不同产地蒙古黄芪原药材的质量状况,为蒙古黄芪药材的质量控制研究提供了新思路。The HPLC-ELSD method was used in the content determination of astragaloside, astragaloside Ⅰ, astragaloside Ⅱ and astragaloside Ⅲ in Mongolia Radix Astragali（Astragalus membranaceus（Fisch.） Bge. var. mongholicus（Bge.） Hsiao） among 16 batches from various habitats. The DIKMA Diamonsil C18（150 mm × 4.6 mm, 5 μm） was adopted with acetonitrile and water as the mobile phase at a gradient mode program. The flow rate was 1.0 mL·min^-1. And the column temperature was 30℃. The ELSD detector parameters were the drift tube temperature at 90℃, and the air flow rate of 2.8 L·min^-1. The SPSS 16.0 software was used in the cluster analysis of content determination. The results showed that when the injection volume was within the range of 0.093 2-1.02 μg（r = 0.999 5）, 0.789-8.78 μg（r = 0.999 7）, 0.506-3.13 μg（r = 0.999 6）, and 0.016 1-1.38 μg（r = 0.999 2） for astragaloside, astragaloside Ⅰ, astragaloside Ⅱ and astragaloside Ⅲ, respectively, the average recoveries were 97.55%, 98.61%, 99.68%, 98.58% with RSD of 1.2%, 1.3%, 1.3%, 1.2%, respectively. The results of cluster analysis showed that the single using of astragaloside as index was unable to differentiate Mongolia Radix Astragali from various habitats. However, the simultaneous determination of 4 types of astragalosides as indexes can differentiate Mongolia Radix Astragali from various habitats. It was concluded that the method was simple, quick and accurate, which can directly reflect the quality status of Mongolia Radix Astragali from different origins. It also provided new ideas for the quality control of Mongolia Radix Astragali.

关 键 词：蒙古黄芪 黄芪皂苷 产地 HPLC-ELSD 

分 类 号：R284.1[医药卫生—中药学]