热毒宁注射液抗菌及调节免疫活性作用研究  

Study on Antibacterial Activity and Immunomodulation Effect of Re-Du-Ning Injection

作　　者：张帅[1,2,3] 王红梅[1,2,3] 常秀娟[1,2,3] 陈健[1,2,3] 陈春苗[1,2,3] 周军[1,2,3] 王振中[1,2,3] 萧伟[1,2,3] 

机构地区：[1]江苏康缘药业股份有限公司,连云港222001 [2]中药制药过程新技术国家重点实验室,连云港222001 [3]江苏省企业院士工作站,连云港222001

出　　处：《世界科学技术-中医药现代化》2015年第5期1056-1060,共5页Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology

基　　金：科学技术部国家重大新药创制项目(2013ZX09402203):现代中药创新集群与数字制药技术平台;负责人:王振中

摘　　要：目的:探讨热毒宁注射液抗菌及提高免疫力作用。方法:将小鼠随机分为正常组、模型组、双黄连组(7.8 m L·kg-1)和热毒宁注射液高、中、低剂量组(20.30 g·kg-1、10.15 g·kg-1、5.08 g·kg-1),采用体内抗菌实验观察其对细菌感染的小鼠死亡保护作用;将小鼠随机分为正常组、模型组、香菇多糖组(0.19 mg·kg-1)和热毒宁注射液高、中、低剂量组(20.30 g·kg-1、10.15 g·kg-1、5.08 g·kg-1),采用2,4-二硝基氯苯所致小鼠迟发型超敏反应、小鼠血清溶血素方法及碳粒廓清实验观察其免疫调节作用。结果:热毒宁注射液高、中剂量组具有抗菌作用,可降低小鼠死亡率;热毒宁注射液高、中、低剂量组可增强免疫低下小鼠巨噬细胞的吞噬能力,能增加小鼠溶血素的生成,增强免疫低下小鼠迟发型的超敏反应。结论:热毒宁注射液具有抗菌,增强小鼠非特异性免疫、体液免疫和细胞免疫作用等免疫调节作用。This study was aimed to discuss the antibacterial and immunomodulation effect of Re-Du-Ning（RDN） injection. Mice were randomly divided into the normal group, model group, Shuang-Huang-Lian（SHL） group（7.8 mL·kg^-1）, RDN high dose group（20.30 g·kg^-1）, middle dose group（10.15 g·kg^-1）, and low dose group（5.08 g·kg^-1）, in order to observe the death protective effect of mice with bacterial infection on antibacterial experiment in vivo. Mice were randomly divided into the normal group, model group, Xiang-Gu Duo-Tang（XGDT） group（0.19 mg·kg^-1）, RDN high dose group（20.30 g·kg^-1）, middle dose group（10.15 g·kg^-1）, and low dose group（5.08 g·kg^-1）. The 2, 4-dinitrochlorobenzene was used to induce delayed hypersensitivity. Immunomodulation was observed by the content of serum hemolysin and the carbon particle clearance index. The results showed that the RDN high dose group and middle dose group had antibacterial effect, which reduced the mortality of mice. The RDN high dose, middle dose and low dose group can enhance the phagocytosis of macrophage in immunosuppressive mice, increase the formation of hemolysin, and strengthen delayed hypersensitivity reaction among immunocompromised mice. It was concluded that RDN injection had antibacterial effect. Its immunomodulation effect was through the enhancing of non-specific immunity, humoral immunity and cellular immunity of mice.

关 键 词：热毒宁 抗菌 体液免疫 细胞免疫 非特异性免疫 

分 类 号：R285.5[医药卫生—中药学]