斑鸠菊果实中总黄酮的提取工艺研究  

Study on Extraction Conditions of Total Flavonoids from Fruit of Vernonia esculenta Hemsl.

作　　者：孙美利 李蕾[1,2,3] 王和宇[1,2,3] 张晶[1] 徐暾海[2,3,4] 刘铜华[2,3] 

机构地区：[1]吉林农业大学中药材学院,长春130118 [2]北京中医药大学中医养生学教育部重点实验室,北京100102 [3]北京中医药大学中医养生学北京市重点实验室,北京100102 [4]北京中医药大学中药学院,北京100102

出　　处：《世界科学技术-中医药现代化》2015年第5期1108-1112,共5页Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology

基　　金：北京中医药大学国际科技合作项目(2012DFG31550):南非传统草药抗糖尿病活性成分筛选及机制研究;负责人:刘铜华;北京中医药大学创新团队项目(2011-CXTD-19):中医药干预糖尿病及其并发症研究团队;负责人:刘铜华

摘　　要：目的:优选斑鸠菊果实中总黄酮的最佳提取工艺。方法:采用分光光度法,通过单因素实验考察乙醇浓度、提取次数、提取时间以及液料比对斑鸠菊果实中总黄酮提取率的影响。结果:最佳提取条件为乙醇浓度70%、提取4次,每次1 h、料液比为1:20。结论:采用最佳提取条件,得到的斑鸠菊果实中总黄酮含量为103.55 mg·g-1。Spectrophotometry and single factor test were used in the investigation of effects on extraction results of total flavonoids from the fruit of Vernonia esculenta Hemsl. from aspects of ethanol concentration, frequency of extraction, length of extraction time, and the solid-liquid ratio. The extraction conditions were optimized by orthogonal test. The results showed that the optimum extraction conditions were ethanol concentration of 70%, extracted for 4 times, 1 h for each time, and the solid-liquid ratio of 1：20. Under these extraction conditions, the content of total flavonoids in the fruit of Vernonia esculenta Hemsl. was 103.55 mg·g^-1.

关 键 词：斑鸠菊 总黄酮 正交试验 分光光度法 

分 类 号：R284[医药卫生—中药学]