中脑星形胶质细胞源性神经营养因子蛋白表达水平与乙型肝炎病毒慢性感染后疾病进展的相关性  

Association between expression level of mesencephalic astrocytederived neurotrophic factor protein and disease progression in patients with chronic hepatitis B virus infection

作　　者：叶娇娇 马双双[1] 郜玉峰[1] 沈玉君[2] 沈玉先[2] 

机构地区：[1]安徽医科大学第二附属医院肝病科,安徽省合肥市230601 [2]安徽医科大学基础医学院,安徽省合肥市230032

出　　处：《世界华人消化杂志》2015年第22期3532-3537,共6页World Chinese Journal of Digestology

基　　金：安徽省教育厅自然科学基金资助项目;No.KJ2014A107;国家自然科学基金资助项目;No.81372576~~

摘　　要：目的:探索中脑星形胶质细胞源性神经营养因子(mesencephalic astrocyte-derived neurotrophic factor,MANF)在乙型肝炎病毒(hepatitis B virus,HBV)慢性感染后不同疾病进展患者外周血中蛋白表达水平的差异及其与临床特征的相关性.方法:采用酶联免疫吸附方法检测正常健康对照(healthy controls,HC)、乙型肝炎病毒携带者(asymptomatic hepatitis B virus surface antigen carriers,ASC)、慢性乙型肝炎(chronic hepatitis B,CHB)患者、乙型肝炎后肝硬化代偿期(compensatory liver cirrhosis,CLC)患者及乙型肝炎后肝硬化失代偿期(decompensated liver cirrhosis,DLC)患者外周血中MANF蛋白的表达水平,分析其在不同阶段慢性HBV感染者中的差异.结果:ASC组、CHB组、CLC组、DLC组和H C组5组之间M A N F的表达差异有统计学意义,F=7.391,P=0.00;进一步进行两两组间分析显示,与HC组、ASC组和CHB组相比较,CLC及DLC组MANF蛋白的表达水平差异均有统计学意义(P<0.01),但HC组与ASC组、CHB组之间两两比较差异均无统计学意义.MANF蛋白的表达水平在不同水平乙型肝炎表面抗原分组(<1500 IU/m L,1500-20000 IU/m L和>20000 IU/m L)之间的差异具有统计学意义(F=9.420,P=0.000);M A N F蛋白的表达水平在不同水平的谷草转氨酶和总胆红素组的差异具有统计学意义(F=3.188,P=0.045;t=2.867,P=0.005);M A N F蛋白的表达水平在不同水平的谷丙转氨酶分组、HBV DNA分组和E抗原状态等分组中未见明显统计学差异.结论:MANF蛋白表达水平下降可能与HBV慢性感染后的疾病进展相关.AIM：To investigate the expression level of endoplasmic reticulum stress related mesencephalic astrocyte-derived neurotrophic factor（MANF） protein and assess its clinical significance in patients with chronic hepatitis B virus（HBV）infection.METHODS：The expression levels of MANF protein in peripheral blood plasma was measured by ELISA in asymptomatic hepatitis B virus surface antigen（HBsAg） carriers（ASC）,chronic hepatitis B（CHB）,compensatory liver cirrhosis（CLC） and decompensated liver cirrhosis（DLC） patients after chronic HBV infection and normal healthy controls（HC）.The relationship between MANF protein expression and different stages of chronic HBV infection was analyzed.RESULTS：The expression levels of MANF protein among the HC,ASC,CHB,CLC,and DLC groups had a statistically significant difference（F = 7.391,P = 0.00）.Further data analysis showed that the expression levels of MANF protein in the CLC and DLC groups had a statistically significant difference compared with the HC,ASC and CHB groups,but there was no significant difference between the HC,ASC and CHB groups.The expression levels of MANF protein had a statistically significant difference in the three groups of patients stratified by HBsAg level（〈 1500 IU/mL,1500-20000 IU/mL and 〉 20000 IU/mL）（F = 9.420,P = 0.000）.The difference in MANF protein expression levels was statistically significant between patients with different levels of aspartate aminotransferase and total bilirubin,but there was no statistical difference between patients with different levels of alanine aminotransferase,HBV DNA,or between hepatitis B e antigen（HBeAg） positive group and HBeAg-negative group.CONCLUSION：The expression levels of MANF protein are negatively associated with the disease progression in patients with chronic HBV infection.

关 键 词：中脑星形胶质细胞源性神经营养因子 内质网应激 乙型肝炎病毒 疾病进展 

分 类 号：R512.62[医药卫生—内科学]