牛磺胆酸钠转运蛋白在肝癌大鼠肝组织中的表达状况  

Sodium/taurocholate cotransporting polypeptide expression in hepatocellular carcinoma in rats

作　　者：张孟瑜[1] 王洁萍[2] 夏先明[1] 

机构地区：[1]泸州医学院附属医院肝胆外科,四川省泸州市646000 [2]泸州医学院附属医院康复科,四川省泸州市646000

出　　处：《世界华人消化杂志》2015年第22期3587-3591,共5页World Chinese Journal of Digestology

基　　金：四川泸州市科技局课题基金资助项目;No.2013-s-48~~

摘　　要：目的:本研究通过建立肝癌大鼠模型,以牛磺胆酸钠转运蛋白(sodium/taurocholate cotransporting polypeptide,Ntcp)为研究对象,初步了解Ntcp在肝癌大鼠中的表达状况,以期能找到新的手段和方法进行肝癌的治疗.方法:取Wistar大鼠(♂、体质量160 g±8 g)60只,随机分为2组,每组30只:普通饮食对照组(简称对照组)予以普通饮食、二乙基亚硝胺饮食实验组(简称实验组)予以含二乙基亚硝胺饮水.经过20 wk,建立肝癌大鼠模型,取对照组和实验组肝脏组织:(1)石蜡切片后用免疫组织化学SP(streptavidin-perosidase)法检测两组模型肝脏组织的Ntcp蛋白表达的强度;(2)Western blot法检测两组模型肝脏组织的Ntcp蛋白表达的强度.结果:免疫组织化学SP法及Western blot法结果均显示,实验组Ntcp表达阳性率高于对照组Ntcp表达阳性率(25.7%),他们之间的表达差异有统计学意义(?2=10.62,P<0.05).结论:实验组大鼠的Ntcp蛋白的表达较对照组增多,提示我们在肝癌的治疗中,Ntcp有可能成为新的治疗靶点和方向.AIM：To detect the expression of sodium/taurocholate cotransporting polypeptide（Ntcp）in hepatocellular carcinoma in rats.METHODS：Sixty Wistar rats were randomly divided into a control group and an experimental group,with 30 rats in each group.The control group was fed an ordinary diet,and the experimental group was fed diethylnitrosamine for 20 wk to induce primary liver cancer.After successful induction,liver tissues were taken to detect the expression of Ntcp protein by immunohistochemistry and Western blot.RESULTS：Both immunohistochemistry and Western blot analysis showed that the expression of Ntcp protein was significantly higher in the experiment group than in the control group（P〈 0.05）.CONCLUSION：The expression of Ntcp protein increases significantly in primary liver cancer in rats,which suggests that drugs targeting Ntcp may provide a new treatment method for primary liver cancer.

关 键 词：Ntcp基因 肝癌 表达 

分 类 号：R735.7[医药卫生—肿瘤]