SD大鼠长期高糖高脂复合饮酒诱发慢性高血压的外周血基因表达谱研究  

The Whole Genome Gene Expression Profile of Peripheral Blood by Microarray Analysis Technique in Hypertension SD Rats Induced by Long-term High-fat & High-sugardiet Combination with Alcohol-drinking

作　　者：季建伟[1] 吕圭源[2] 高建莉[2] 李波[1] 苏洁[2] 吴玉兰[1] 陈素红[1] 

机构地区：[1]温州医科大学,浙江温州325035 [2]浙江中医药大学

出　　处：《浙江中医药大学学报》2015年第6期423-429,共7页Journal of Zhejiang Chinese Medical University

基　　金：国家自然科学基金面上项目(81274123;81374003);浙江省重点实验室(2012E10002)~~

摘　　要：[目的]采用全基因组微阵列技术(4x44K,Agilent Technologies)研究高糖高脂复合饮酒因素对SD大鼠外周血中m RNA基因表达谱的影响,探讨该复合因素诱导大鼠高血压的潜在机制。[方法]采用高糖高脂复合饮酒喂养SD大鼠8个月诱导大鼠慢性高血压,正常对照组喂养普通饲料和饮水。测定造模前后大鼠的血压和血脂水平,随后每组随机抽取大鼠外周血样,进行基因表达谱分析。[结果]与正常组比较,高糖高脂复合饮酒能显著升高收缩压和舒张压,增加血浆中的TC水平。基因芯片结果显示,差异表达基因共有2209个,其中上调基因560个,下调1649个。对差异基因进行GO分析,结果表明高糖高脂复合饮酒模型组上调细胞结合、免疫调节等相关基因,下调细胞外基质、突触等相关基因。对差异基因进一步进行pathway分析,发现高糖高脂复合饮酒模型组上调黏附因子和抗原加工递呈等43条通路,下调花生四烯酸代谢和脂类代谢等13条通路。[结论]高糖高脂复合饮酒诱导SD大鼠血压升高可能与激活大鼠免疫应答、血管炎症、黏附因子以及抑制脂类代谢等功能有关。[Objective]Exploring the underlying mechanisms for multiple factor（high-fat ＆ high-sugar diet combination with alcohol-drinking） induced hypertension in SD rats by Microarray（4x44K, Agilent Technologies） analysis. [Methods]Ten SD rats were received high fat and sugar diet combination with alcohol-drinking was taken for 8 months to induce hypertension, and the other ten SD ＇rats were received normal diet and water as control. The blood pressure and lipid level were determined before and after model, peripheral blood samples in each group were randomly selected for Microarray analysis. [Results]High-fat and sugar diet combination with alcohol-drinking can significantly increase the systolic and diastolic blood pressure and TC level compa^d with the normal group. Microarray analysis revealed that a total number of 2209 genes were differentially expressed, 560 were upregulated and 1649 were downregulated. GO analysis showed that the top-enrichment score of up-regulate genes in model group was associated with binding and regulation of immune response, and that the top-enrichment score of down-regulating genes in model group was extracellular region and synapse etc. Pathway analysis indicated that 43 functional pathways such as Cell adhesion molecules（CAMs） and Antigen processing and presentation were up- regulated, and 13 pathways such as Arachidonic acid metabolism and Ether lipid metabolism were down-regulated. [Conclusions]High-fat and sugar diet combination with alcohol-drinking induced hypertension of SD rats, is related to activating immune response, vascular inflammation and adhesion molecules etc, and inhibits lipid metabolism.

关 键 词：高血压 高糖高脂 长期饮酒 基因表达谱 全基因组微阵列技术 

分 类 号：R331[医药卫生—人体生理学]