水稻类病斑突变体LM1基因的图位克隆  

Map-based Cloning of LM1 Gene of Lesion Mimic Mutant in Rice( Oryza sativa L.)

作　　者：秦江曼 李战朋[1] 张治国[1] 路铁刚[1] 吴金霞[1] 

机构地区：[1]中国农业科学院生物技术研究所,北京100081

出　　处：《中国农业科技导报》2015年第4期8-14,共7页Journal of Agricultural Science and Technology

基　　金：中国-巴西作物生物技术与种子资源联合研究项目(2014DFA31550)资助

摘　　要：类病斑坏死突变体(lesion mimic mutant,LMM)是一类表型类似于植物过敏反应的突变体,对植物抵御外界病原菌浸染的抗病机理研究具有重有意义。以水稻突变体库中类病斑突变体lm1为材料,经图位克隆获得突变基因LM1,同时测定了相关生理指标。分析结果表明:与野生型相比,突变体叶片细胞中的丙二醛(MDA)积累水平明显升高,而叶绿素含量、Fv/Fm却显著低于野生型。遗传分析及精细定位显示,lm1突变体表型受一对隐性核基因LOC_Os12g16720控制,并将该基因命名为LM1,LM1位于水稻第12号染色体短臂的着丝粒附近,是SPL1的等位基因,编码细胞色素P450单加氧酶;LM1突变导致其功能缺失,使植物体内活性氧得到累积,引发细胞程序性死亡并诱导病斑形成。Lesion mimic mutant （LMM） was a class of plant mutant, similar to allergic reaction, and was of significant importance for studying the mechanism of plant pathogens against outside dissemination. In this study, a lesion-mimic mutant derived from Rice mutant library, was designated lml. Physiological indicators was analyzed and measured between mutant and wild-type. Compared with wild-type plants, malondialdehyde （MDA） accumulation in the mutant plant leaf cells was significantly increased, and the chlorophyll content, Fv/Fm ratio in mutant plants were significantly decreased compared with the wild-type plants. Genetic analysis indicated that the lml mutant was controlled by a single recessive gene. LM1 gene was isolated by map-based cloning method. The LM1 gene was narrowed on the short arm of chromosome 12 and it was controlled by LOC_Os12g16720. Protein sequence showed that LM1 encodes a P450 monooxygenase, which was allele with the SPL1 gene. The P450 monooxygenase was an essential enzyme involving in plant serotonin biosynthesis pathway.

关 键 词：水稻 类病斑坏死突变体 图位克隆 P450单加氧酶 

分 类 号：Q78[生物学—分子生物学]