紫花苜蓿两种再生体系的优化及比较  

Optimization and Comparison of Two Regeneration System of Alfalfa( Medicago sativa L.)

作　　者：王成龙[1,2] 周美亮[2] 董雪妮[1,2] 唐益雄[2] 邵继荣[1] 吴燕民[2] 

机构地区：[1]四川农业大学生命科学学院,四川雅安625001 [2]中国农业科学院生物技术研究所,北京100081

出　　处：《中国农业科技导报》2015年第4期53-61,共9页Journal of Agricultural Science and Technology

基　　金：国家973计划项目(2014CB138701)资助

摘　　要：紫花苜蓿高频再生体系的建立有助于促进其遗传转化的研究。以陇东、阿尔冈金以及甘农4号紫花苜蓿为实验材料,比较了体细胞胚胎和丛生芽再生途径对于紫花苜蓿再生周期及频率的影响。并通过利用不同外植体、不同植物生长调节剂配比,以实现对这两种再生途径的进一步优化。实验结果表明:在三个苜蓿品种中,甘农4号的再生效果较好,下胚轴的再生频率较高,在愈伤诱导阶段添加2 mg/L 2,4-D、1 mg/L KT,胚状体诱导阶段添加1 mg/L KT、0.5 mg/L NAA可以达到较好的再生效果;在培养基中添加1 mg/L 6-BA、1 mg/L TDZ,则直接诱导丛生芽的效果最好;对比发现,丛生芽再生途径显示出较强的优越性,其再生时间仅10周,是紫花苜蓿再生体系建立的一种可选方式。The establishment of alfalfa high frequency regeneration system is helpful to promote the studies on its genetic transformation. Taking Longdong, Algonquin and Gannong 4 alfalfa （Medicago sativa L.） as experimental materials, this study compared the effects by somatic embryo regeneration and multiple shoots clumps regeneration on alfalfa regeneration cycle and frequency. Meanwhile, different explants and growth regulators were matched to realize further optimization of these 2 regeneration pathways. The results showed that the regeneration effect of Gannong 4 was better 3 alfalfa species and hypocotyl was higher. In callus induction phase adding 2 mg/L 2,4-D and 1 mg/L, in embryoid induction phase adding 1 mg/L KT and 0.5 mg/L NAA, better regeneration effect could be achieved. Adding lmg/L 6-BA and lmg/L TDZ in culture medium could get the best regeneration via multiple shoots. Regeneration via muhiple shoots clumps pathway had shown excellent superiority. Its regeneration time was only 10 weeks, which was an alternative way for the establishment of alfalfa regeneration system.

关 键 词：紫花苜蓿 胚胎再生途径 丛生芽诱导 

分 类 号：Q943.1[生物学—植物学] S551.7[农业科学—作物学]