甲型H1N1流感监测及其HA1基因特性分析,以2013~2014年新余市为例  

Virological Surveillance and Analysis on the Characteristics of HA1 Gene of Influenza a Virus H1N1, Case study of Xinyu City from 2013 to 2014

作　　者：潘虹[1] 刘瑞弘[1] 文奇[1] 刘琴[1] 周银古 熊英[2] 

机构地区：[1]新余市疾病预防控制中心,新余338000 [2]江西省疾病预防控制中心,新余338000

出　　处：《基因组学与应用生物学》2015年第2期247-251,共5页Genomics and Applied Biology

基　　金：国家重点基础研究发展计划973项目(2014CB138302)资助

摘　　要：因HA基因的头部重链区（HA1）是流感病毒的抗原位点和受体结合位点,所以了解流感病毒的HA1基因特性可以评估当前WHO推荐的疫苗株的预防效果。本文选取新余市2013-2014年通过狗肾传代细胞（MDCK）培养分离到的H1N1流感毒株12株,提取病毒RNA,反转录-聚合酶链反应（RT-PCR）扩增HA1基因片段。对序列分析发现2013-2014年新余市甲型H1N1流感病毒HA1基因序列与疫苗推荐株有较高的同源性,核苷酸同源性97.9%-98.5%。2013年的6株毒株HA1区与疫苗株比较,氨基酸变异位点7-11个,其中3株有2个变异位点在不同的抗原决定簇区。而2014年毒株单独集中在进化树的一支。以上提示,目前流感疫苗对2014年的流感人群仍有保护作用,对2013年的部分流行的H1N1预防效果有限;甲型H1N1爆发流行的可能性很大,应加强防控。Due to head heavy chain region（HA1） of the HA gene are antigenic sites and receptor binding sites of influenza virus. To understand the characteristics of HA1 genes of influenza virus that can evaluate the preventive effect of the current vaccine strain recommend by WHO. 12 strains of influenza A virus H1N1 isolated in Xinyu from 2013 to 2014 were cultured in MDCK cell in this article. Viral RNA were extracted and HA1 gene were amplified by using RT-PCR. The nucleotide sequences were determined and analyzed. The results showed that there was high homology of HA1 between influenza A virus H1N1 isolates from Xinyu City from 2013 to 2014 and the vaccine strain recommended by WHO, the identity were 97.9%-98.5% in nucleotides sequence. Between 6strains of influenza A virus H1N1 isolated in Xinyu from 2013 and vaccine strain, 7-11 amino acids changed, 2mutated sites of 3 strains in different antigenic determinant sites. The strains from 2014 existed in a branch of the evolutionary tree. The above prompted that the vaccine produced by influenza a virus H1N1 vaccine strain recommended by WHO had protective effect to H1N1 of 2014, had terminate protective effect to part of influenza H1N1 in 2013. The outbreak of influenza H1N1 is possible. We should strengthen prevention and control.

关 键 词：H1N1甲型流感病毒 血凝素基因 进化分析 

分 类 号：R440[医药卫生—诊断学] R511.7[医药卫生—临床医学]