重度肥胖患者肠道内机会性致病菌Escherichia coli微多样性分析  

Micro-diversity Analysis of Opportunistic Pathogen Escherichia coli Isolated from a Severely Obese Human Gut

作　　者：赵梅[1] 王睿瑞[2] 徐佳[2] 方超[1] 赵宇峰[1,2] 

机构地区：[1]上海交通大学系统生物医学研究院,上海201100 [2]上海交通大学生命技术学院微生物代谢国家重点实验室,上海201100

出　　处：《基因组学与应用生物学》2015年第2期252-256,共5页Genomics and Applied Biology

基　　金：国家科技重大专项(2012ZX10005001-009);国家自然基金面上项目(81273720)和国家自然基金重点项目(31330005)共同资助

摘　　要：本研究利用志贺氏菌显色培养基和ERIC-PCR指纹图谱技术,从一名重度肥胖患者肠道内分离出19种不同ERIC类型的分离物。选取不同ERIC类型的代表菌株进行16S r RNA基因分子鉴定并构建系统发育树,发现19种代表菌株的16S r RNA基因和埃希氏菌属(Escherichia)、志贺氏菌属(Shigella)的相似性达到99%。经生理生化反应鉴定,19种代表菌株均为肠杆菌科的肠埃希氏菌(Escherichia coli)。体外生物学分析发现19种E.coli代表菌株在生化特性、耐药性、毒性方面有明显差异。本研究表明来自一名重度肥胖患者肠道内同一种群的E.coli在菌株水平上具有丰富的微多样性,不同ERIC类型的E.coli在生理生化特性上存在较大的差异,且部分菌株具有潜在致病性。In this research we obtained 19 isolates with different ERIC types through selective medium plate and ERIC-PCR finger-printing technology. By 16 S rRNA gene sequencing and biochemical reaction identification, 19 representative isolates were all identified to be Escherichia coli. Analyzing the physiological and biochemical characteristics of 19 representative isolates E. coli in vitro, we found that there were obviously difference in the biochemical properties, toxicity and drug-resistance. In summary, this research showed that E. coli isolated from an obese human gut have a rich micro-diversity. Different genotypes have different biological characteristics and some isolates have potential pathogenicity.

关 键 词：机会性致病菌 肠埃希氏菌 微多样性 肥胖症 

分 类 号：R589.2[医药卫生—内分泌]