甘草黄酮对长期大强度运动小鼠心肌损伤的保护作用  

Protective Effect of Glycyrrhizic Flavone on Myocardial Injury Induced by Long-term Exhaustive Exercised Rats

作　　者：李敏华[1] 唐健[1] 王春亮[1] 

机构地区：[1]广西师范大学体育学院,桂林541004

出　　处：《基因组学与应用生物学》2015年第2期290-295,共6页Genomics and Applied Biology

基　　金：广西教育厅科学研究项目(201106LX531)资助

摘　　要：为了探讨甘草黄酮对长期大强度运动小鼠心肌损伤的保护作用机制。本研究选用50只昆明雄性小鼠为研究对象,以每组10只分组,分为安静对照组(A组)、运动训练组(B组)、运动低剂量给药组(C组)、运动中剂量给药组(D组)、以及运动高剂量给药组(E组)。运动低剂量给药组(C组)、运动中剂量给药组(D组)、运动高剂量给药组(E组)训练前分别灌服剂量为5 g/kg/d、10 g/kg/d、15 g/kg/d黄酮溶液;除安静对照组外,运动组小鼠进行为期6周的大强度游泳训练。测定小鼠各组小鼠血清CK-MB、c Tn I,心肌组织中GSH-PX、SOD、CAT、MDA、NOS、NO、Na+/K+-ATP、Ca2+/Mg2+-ATP。结果表明:与安静对照组比较,运动训练组CK-MB、c Tn I、MDA、NO、NOS升高,GSH-PX、SOD、CAT、Na+/K+-ATP、Ca2+/Mg2+-ATP降低。与运动训练组比较,运动低剂量给药组、运动中剂量给药组、运动高剂量给药组CK-MB、c Tn I、MDA、NO、NOS降低,GSH-PX、SOD、CAT、Na+/K+-ATP、Ca2+/Mg2+-ATP升高;与运动低剂量给药组比较,运动高剂量给药组CK-MB、c Tn I、GSH-Px、SOD、Na+/K+-ATP、Ca2+/Mg2+-ATP升高,MDA降低。本研究提示,长期大强度运动导致机体心肌损伤,甘草黄酮可提高机体心肌组织中抗氧酶、ATPase酶的活性,抑制自由基生成、NOS活性,减少NO的生成,维持细胞膜内外Na+、K+、Ca2+、Mg2+的分布平衡,对大强度运动造成的心肌损伤具有保护作用,效果以高剂量黄酮最佳。In order to explore the protective mechanism of glycyrrhizic flavone on myocardial injury induced by long-term exhaustive exercised rats, we employed 50 male Kunming rats as the experimental materials that were randomly grouped into five groups by 10 rats each, which were sedentary control group（group A）, trained group（group B）, low dosage plus trained group（group C）, medium dosage plus trained group（group D）, and high dosage plus trained group（group E）. All rats of the dosage-given group, group C, D and E were fed with 5 g/kg/d, 10 g/kg/d and 15 g/kg/d of glycyrrhizic flavone solution respectively in prior to doing experimental exercise for lasting six-week intensive swimming exercises except for the sedentary control all experimental rats in this research were determined to measure the CK-MB and cTnI of rats＇ serum, as well as GSH-PX, SOD, CAT, MDA, NOS, NO,Na^＋/K^＋-ATP, and Ca2＋/Mg^2＋-ATP of myocardial tissues. Results exhibited that the parameters of CK-MB, cTnI,MDA, NO, and NOS in treated groups were increased, whereas the parameters of GSH-PX, SOD, CAT,Na^＋/K^＋-ATP, and Ca^2＋/Mg^2＋-ATP were de creased in contrast of sedentary control. However, the parameters of CK-MB, cTnI, MDA, NO, and NOS were decreased, whereas GSH-PX, SOD, CAT, Na^＋/K^＋-ATP, and Ca^2＋/Mg^2＋-ATP were increased in low dosage plus trained group, medium dosage plus trained group, and high dosage plus trained group compared to the control. But by comparing with low dosage plus trained group, the parameters of CK-MB, cTnI, GSH-Px, SOD, Na^＋/K^＋-ATP, and Ca^2＋/Mg^2＋-ATP were increased and MDA was decreased. In this research we thought that the long-term intensive exercises might result in the myocardial injury of organism, but the glycyrrhizic flavone could enhance the antioxidant enzymes in myocardial tissues and inhibit the generation of NOS activity, which may maintain the balanced distribution of Na^＋, K^＋, Ca^2＋, Mg^2＋.Therefore, we proposed that the treatment with high dosage

关 键 词：甘草黄酮 昆明鼠 大强度运动 心肌损伤 保护作用 

分 类 号：R285.5[医药卫生—中药学]