大鼠前脑不同缺血时间后再灌注损伤模型的构建与评价  

Establishment and evaluation of ischemia-reperfusion injury models by different ischemia time of rats

作　　者：王凤[1] 黄秀清[1] 王萍萍[1] 林丽琳[1] 陈秀娇[1] 张敏[1] 

机构地区：[1]莆田学院基础医学院,福建351100

出　　处：《齐齐哈尔医学院学报》2015年第22期3286-3288,共3页Journal of Qiqihar Medical University

基　　金：2013年福建省大学生创新创业训练计划项目(201311498017);2013年度莆田学院大学生创新创业训练计划项目(CXCY1303)

摘　　要：目的建立缺血再灌注损伤wistar大鼠模型。方法本实验将50只wistar大鼠随机平分成正常组,假手术组,缺血10 min组,缺血20 min组,缺血30 min组。采用双侧颈总动脉夹闭法,夹闭一定时间后再通,重复3天。通过HE染色技术观察大鼠海马组织的损伤情况,计数400倍光镜视野下的海马组织细胞数目以及脑重量和脑系数进行比较分析。结果缺血20 min组大鼠的海马变性坏死明显,缺血10 min组变性程度较轻微,缺血30 min组细胞溶解,结构不清,正常组和假手术组均无上述改变。结论双侧颈总动脉夹闭法有效地构建不同缺血时间后再灌注损伤模型,具有操作简单,安全性大,重复性高的优点,为今后缺血再灌注损伤的实验研究提供有效的方法及病理形态学依据。Objective To establish ischemia-reperfusion injury models of wistar rats. Methods This experiment divided 50 rats into 5 groups randomly and equally. There were a normal group,a control group and 3model groups by different ischemia time（ 10 min,20min and 30min）. By adopting bilateral carotid artery occlusion,and keeping a period time,then making the recanalization,repeated for 3 days. Used the HE staining technique to observe the damage of the hippocampal tissue of rats,counted the number of hippocampus cells under the microscope with 44 times light field of vision. Weighted the brain and calculate the coefficient of the brain,then made a comparison. Results The coefficient cells of the group by 20 min ischemia time was denaturation and necrosis obviously,the cells of the 30 min one was dissolved and the structure was not clear. The normal group and the control group didn＇t have the above alteration. Conclusions Bilateral carotid artery occlusion could establish the ischemia-reperfusion injury models by different ischemia time of rats effectively. There are more advantages,such as operating easily,more security,higher repeatability. It will provide effective methods of the experimental study and the pathological morphology in the future.

关 键 词：缺血再灌注损伤 动物模型 海马细胞 缺血时间 模型评价 

分 类 号：R657.3[医药卫生—外科学]