褪黑素对大鼠生长及脂肪代谢、分配的影响  

Effects of Melatonin on Growth,and Lipometabolism and Partition of Fat in Rat

作　　者：王林枫[1] 柳如意 杨改青[2] 朱河水[1] 王月影[1] 韩立强[1] 贾少丹[1] 杨国宇[1] 

机构地区：[1]河南农业大学牧医工程学院农业部动物生化与营养重点试验室,郑州450002 [2]河南农业大学现代试验技术与管理中心,郑州450002

出　　处：《动物营养学报》2015年第8期2456-2465,共10页CHINESE JOURNAL OF ANIMAL NUTRITION

基　　金：国家"973"计划项目(2011CB100802);河南省教育厅科学技术研究重点项目(14B230015)

摘　　要：本试验旨在研究褪黑素(MT)对动物生长及脂肪代谢、分配的影响。选择日龄、体重接近的SD雄性大鼠50只,随机平均分为5组,每组10只,5组分别为正常饲粮(ND)、正常饲粮+褪黑素(ND+MT)、脂肪添加(FS)、脂肪添加+低剂量褪黑素(FS+LMT)和脂肪添加+高剂量褪黑素(FS+HMT)组。FS组在正常饲粮中添加10%的猪油,ND+MT、FS+LMT、FS+HMT组除饲喂特定饲粮外,分别灌服10、2、20 mg/kg BW的MT溶液。试验期60 d。结果表明,与ND组相比,ND+MT组日均采食量、平均日增重、肝脏重显著下降(P<0.05);肝脏长链脂肪酸含量、总胆固醇含量、大网膜重、肾周脂肪重、附睾脂肪重均显著降低(P<0.05)。较FS组,FS+LMT组日均采食量、平均日增重、肝脏长链脂肪酸含量、大网膜重、附睾脂肪重降低,肝脏指数显著升高(P<0.05);FS+HMT组的大网膜重、附睾脂肪重较显著降低(P<0.05)。较ND组,FS组肾周脂肪重显著降低(P<0.05),而附睾脂肪重则显著增加(P<0.05)。综上,MT可以降低大鼠的生长速度,减少大鼠肝脏内脂肪的含量,改变脂肪在体内的分配。The present study was aimed to investigate effects of melatonin on growth,and lipometabolism and partition of fat in rat.Fifty SD rats with similar age and body weight were randomly divided into 5 groups（10 rats per group）,which were normal diet（ND）,normal diet with melatonin（ND ＋ MT）,fat supplementation（FS）,fat supplementation with lowdose melatonin（FS ＋ LMT） and fat supplementation with high dose melatonin（FS ＋ HMT） group.FS group was refer to adding 10% lard into normal diet.Except of special diets,rats in ND ＋ MT,FS ＋ LMT and FS ＋ HMT groups were administrated orally with 10,2,20 mg/kg BWof melatonin solution every day,respectively.The experiment lasted for 60 days.The result showed as follows： compared with ND group,the contents of hepatic long chain fatty acids（LCFA）,total cholesterol,and average daily gain,average daily feed intake,liver weight,omentum majus fat weight,perirenal fat weight and epididymis fat weight in ND ＋ MT group were decreased significantly（P〈0.05）.Compared with FS group,average daily gain,average daily feed intake,content of hepatic LCFA,omentum majus fat weight and epididymis fat weight in FS ＋ LMT group were significantly decreased（P〈0.05）,while liver index was significantly increased（P〈0.05）.Compared with FS group,omentum majus fat weight and epididymis fat weight in FS ＋ HMT group were significantly decreased（P〈0.05）.Compared with ND group,perirenal fat weight in FS group was significantly decreased（P〈0.05）,and epididymis fat weight in FS group was significantly increased（P〈0.05）.In conclusion,melatonin has a dose-related effect that can reduce the growth rate of rats,decrease the fat content in the liver,and change the distribution of body fat.

关 键 词：褪黑素 大鼠 生长 脂肪分配 

分 类 号：S852.2[农业科学—基础兽医学]