芪卫颗粒干预糖尿病肾病小鼠足细胞损伤的作用研究  

Effect of Qiwei Granules on Renal Podocyte Injury in Diabetic Nephropathy Mice

作　　者：周静鑫[1,2] 孙文[2] 张岩[2,3] 吴丽丽[2,4] 秦灵灵[2,5] 郭璇[2,3] 许光远[2,3] 李林忆[2,3] 段颖[2,6] 郭翔宇[2,3] 吴欣莉[2,3] 刘铜华[2] 

机构地区：[1]北京中医药大学东直门医院东区,北京101121 [2]北京中医药大学教育部中医养生学重点实验室,北京100029 [3]北京中医药大学东方医院,北京100078 [4]北京中医药大学养生学研究所,北京100029 [5]北京中医药大学科技处,北京100029 [6]首都医科大学附属北京同仁医院传统医学科,北京100730

出　　处：《世界科学技术-中医药现代化》2015年第6期1150-1156,共7页Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology

基　　金：北京中医药大学创新团队项目(NO.2011-CXTD-19):中医药防治糖尿病研究团队;负责人:刘铜华

摘　　要：目的:探讨芪卫颗粒干预糖尿病肾病小鼠足细胞损伤的作用机制。方法:28只8周龄KK-Ay小鼠成模后分为模型组和芪卫低剂量组、芪卫中剂量组及芪卫高剂量组,另8只C57BL/6J小鼠设为正常对照组。实验中纪录小鼠一般状况、血糖、24 h尿蛋白定量。治疗10周后检测小鼠肾功能指标血清肌酐及尿素氮,并称取小鼠肾脏,固定后行小鼠肾组织苏木精-伊红(HE)、Masson、过碘酸希夫(PAS)染色,并制作肾母细胞瘤基因(WT-1)免疫组化,结合软件分析计算小鼠肾小球足细胞数。蛋白质印迹法(Western Blot)检测小鼠肾脏中nephrin蛋白表达,实时荧光定量聚合酶链反应(PCR)检测nephrin m RNA的表达。结果:与模型组相比,芪卫颗粒治疗10周后小鼠体质量、血糖、24 h尿蛋白定量及血肌酐明显降低,并有效改善肾小球系膜增生等病理损害,保护足细胞数目。芪卫颗粒治疗后的nephrin蛋白表达量及m RNA表达量均显高于模型组。结论:芪卫颗粒可能通过调节nephrin表达而改善糖尿病肾病小鼠足细胞损伤。This article was aimed to study the effect of Qiwei granules on the podocyte in KK-Ay mice kidney. The 28 8-week-old male KK-Ay mice were randomly divided into the model group, low-dosage, middle-dosage and high-dosage Qiwei granule group. Eight C57BL/6J mice were used as the normal control. The general conditions, blood glucose and 24 h albuminuria were recorded in the experiment. After 10-week treatment, renal indexes including serum creatinine and urea nitrogen were measured. The kidneys of mice were collected and measured. The hematoxylin and eosin（HE）, Masson＇s Trichrome, and periodic acid-Schiff（PAS） were used on renal tissues of mice. The immunohistochemical staining for WT-1 was made. Software analysis was combined in the calculation of renal podocyte amount. Western blot was used in the detection of nephrin protein expressions in the kidney of mice. RT-PCR was used in the detection of nephrin m RNA expression. The results showed that compared with the model group, the body weight, blood glucose, 24 h albuminuria and the serum creatinine were obviously decreased after 10-week treatment of Qiwei granules. It can effectively improve the glomerular mesangial proliferation and preserve the podocyte number. Meanwhile, after the treatment of Qiwei granules, the nephrin protein expression and m RNA expression were obviously higher than the model group. It was concluded that Qiwei granules probably managed nephrin expression to improve the podocyte injury in the diabetic nephropathy of KK-Ay mice.

关 键 词：芪卫颗粒 糖尿病肾病 足细胞损伤 KK-AY小鼠 

分 类 号：R285.5[医药卫生—中药学]