滋补脾阴方药对脾阴虚痴呆大鼠脑内NMDA受体mRNA表达的影响  

Effect of Zi-Bu Pi-Yin Recipe on m RNA Expressions of NMDA Receptor in Different Brain Regions of Spleen-yin Deficiency Alzheimer's Disease Model Rats

作　　者：宫晓洋[1] 战丽彬[2,3] 隋华[4] 梁丽娜[4] 王健[5] 

机构地区：[1]大连医科大学附属第一医院,大连116011 [2]大连医科大学附属第二医院,大连116023 [3]南京中医药大学基础医学院,南京210023 [4]大连医科大学中西医结合基础研究所,大连116044 [5]大连医科大学科技处,大连116044

出　　处：《世界科学技术-中医药现代化》2015年第6期1235-1242,共8页Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology

基　　金：国家自然科学基金委面上项目(30472255):滋补脾阴方药对兴奋性传递中树突棘调控的分子机制研究;负责人:战丽彬;辽宁省高等学校创新团队项目资助(LT2014018):中医"脾主运化;主思藏意"的现代生物学研究;负责人:战丽彬

摘　　要：目的:观察滋补脾阴方药对脾阴虚痴呆大鼠脑内N-甲基-D-天冬氨酸受体(NMDAR)亚单位NR1、NR2A、NR2B m RNA表达的影响。方法:运用逆转录聚合酶链反应(RT-PCR)技术,对NMDAR亚单位NR1、NR2A、NR2B m RNA在各脑区的表达状况作相应的检测。结果:痴呆组以及脾阴虚痴呆组大鼠NMDAR亚单位NR1、NR2A、NR2B m RNA在各脑区表达均明显减弱下调(P<0.05);ZBPYR治疗组NMDAR亚单位NR1、NR2A、NR2B m RNA均明显上调(P<0.05)。结论:使用ZBPYR治疗的大鼠NMDAR m RNA的表达水平在各脑区均明显上调,这表明ZBPYR以提升NMDAR m RNA的方式,使得NMDAR蛋白的含量显著增多,而发挥其抗痴呆作用。This study was aimed to observe the effect of Zi-Bu Pi-Yin Recipe（ZBPYR） on the m RNA expressions of NMDA receptor subunits NR1, NR^2 A, NR^2 B in different brain regions of spleen-yin deficiency Alzheimer＇s Disease（AD） model rats. The levels of NR1, NR^2 A, NR^2 B m RNA expressions were detected by using RT-PCR method. The results showed that the levels of NR1, NR^2 A, NR^2 B m RNA expressions of AD group and spleen-yin deficiency AD group decreased significantly（P〈0.05）. The levels of NR1, NR^2 A, NR^2 B m RNA expressions of ZBPYR treatment group increased significantly（P〈0.05）. It was concluded that the expression levels of NMDAR m RNA in different brain regions of the ZBPYR treatment group increased significantly, which indicated that ZBPYR may up-regulate the protein expressions of NMDAR by increasing the expression levels of NMDAR m RNA, thereby to play the anti-dementia effect.

关 键 词：脾阴虚痴呆 滋补脾阴方药 NR1 NR2A NR2B 

分 类 号：R285.5[医药卫生—中药学]