清肠化湿方对实验性结肠炎小鼠IL-6反式信号转导的影响  

Influence of Qing-Chang Hua-Shi Recipe on IL-6 Trans-signaling in Experimental Colitis in Mice

作　　者：赵青春[1] 沈洪[2] 程海波[1] 朱磊[2] 李沐涵[1] 

机构地区：[1]南京中医药大学第一临床医学院,南京210046 [2]江苏省中医院,南京210029

出　　处：《世界科学技术-中医药现代化》2015年第6期1249-1253,共5页Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology

基　　金：国家自然科学基金委面上项目(81373606):健脾补肾;清肠化湿介导MAPK/ERK促进间充质干细胞迁移分化调节免疫;重建溃疡性结肠炎肠粘膜屏障的研究;负责人:沈洪;国家自然科学基金委青年基金项目(81403344):黄芩苷干预PI3K/AKT/NF-κB信号通路对溃疡性结肠炎免疫调节;肠黏膜屏障修复的作用机制研究;负责人:朱磊;国家中医药行业科研专项资助(201407001):提高中医药防治重大疑难疾病临床能力与水平的使用技术研究;负责人:吕玉波;江苏省临床医学科技专项项目资助(BL2014100);负责人:沈洪

摘　　要：目的:观察清肠化湿方对实验性结肠炎小鼠白介素6(IL-6)反式信号转导trans-signaling的影响,初步探讨该方治疗溃疡性结肠炎(UC)作用机制是否与调控IL-6 trans-signaling有关。方法:TNBS法制备小鼠结肠炎模型,药物干预后,ELISA法检测可溶性白细胞介素6受体(s IL-6R)含量,实时荧光定量PCR法检测IL-6、糖蛋白130(gp130)m RNA相对表达水平,Western Blot法观察结肠黏膜IL-6、gp130蛋白的表达情况。结果:模型组小鼠s IL-6含量、IL-6及gp130 m RNA和蛋白表达水平较正常小鼠明显增高。清肠化湿方可以降低实验性结肠炎小鼠结肠s IL-6水平(P<0.01),降低IL-6及gp130m RNA和蛋白表达水平(P<0.01)。结论:清肠化湿方对小鼠实验性结肠炎发挥良好抗炎效应,可能与调控IL-6 trans-signaling有关。This study was aimed to observe the influence of Qing-Chang Hua-Shi Recipe（QHR） on IL-6 transsignaling in experimental colitis mice, in order to initially explore the possible mechanisms of QHR for ulcerative colitis（UC）. TNBS/ethanol was used in the establishment of colitis mice model. After intervention of medication, ELISA was used in the detection of soluble Interleukin-6 receptor（s IL-6R）. Real-time PCR was used to detect the m RNA expression level of IL-6 and glycoprotein 130（gp130）. Western blot was used in the observation of protein expression of IL-6 and gp130 in the colonic mucosa. The results showed that the level of s IL-6R, the m RNA and protein expression of IL-6 and gp130 in the model group were significantly higher than that in the control group. QHR was able to reduce the s IL-6R level（P〈0.01）, decreased the m RNA and protein expression of IL-6 and gp130（P〈0.01） in the colon tissues among experimental colitis mice. It was concluded that QHR had good antiinflammatory effects on experimental colitis mice. It might be associated with influencing IL-6 trans-signaling.

关 键 词：清肠化湿方 溃疡性结肠炎 白细胞介素-6 SIL-6R GP130 trans-signaling 

分 类 号：R285.5[医药卫生—中药学]