胶原羟基磷灰石涂层人工机械瓣膜实验研究  

Artificial mechanical valve coated with collagen hydroxyapatite：an experimental study

作　　者：程光存[1] 严宇[1] 李春生[1] 韦小勇[1] 严中亚[1] 罗乐[2] 方晓东[3] 陶汝华[3] 

机构地区：[1] 安徽省心血管病研究所,安徽医科大学附属省立医院心脏外科230001 [2] 合肥工业大学 [3] 中科院安徽光学精密机械研究所

出　　处：《中华生物医学工程杂志》2015年第1期14-19,共6页Chinese Journal of Biomedical Engineering

基　　金：安徽省自然科学基金

摘　　要：目的：通过在胶原包埋羟基磷灰石涂层人工机械瓣膜体外内皮化和体内内皮化比较研究，了解胶原包埋羟基磷灰石涂层人工机械瓣膜支架材料在体内的再细胞化能力，并对支架的性能作全面的验证。方法将传代的犬血管内皮细胞制成细胞悬液接种在胶原包埋羟基磷灰石涂层人工机械瓣膜材料上，置培养箱内培养。静态组胶原包埋羟基磷灰石涂层人工机械瓣膜继续置于37℃孵箱中静态培养2周，动态组胶原包埋羟基磷灰石涂层人工机械瓣膜置于特制动态旋转培养装置中培养2周，测定血管内皮细胞分泌一氧化氮（NO）、前列环素（PGI2）水平。分别将单纯人工机械瓣组、胶原包埋羟基磷灰石涂层人工机械瓣膜组、静态组、动态组，四组瓣膜材料分别植入犬右心房，手术后6周，取出材料，于扫描电子显微镜（SEM）下观察细胞在材料上的附着情况、支架材料在体内的再细胞化能力、内皮细胞层形成情况及观察抗凝特点。结果在动态组材料上3、6、7、12 d血管内皮细胞分泌NO水平（110.92＆#177;6.92、116.62＆#177;6.84、119.45＆#177;5.14、123.52＆#177;4.93）、PGI2水平（19.21＆#177;0.52、29.13＆#177;0.57、26.67＆#177;0.52、21.58＆#177;1.34）显著高于静态组NO水平（81.91＆#177;6.89、81.90＆#177;6.88、83.48＆#177;5.91、84.68＆#177;4.81）、PGI2水平（16.31＆#177;0.55、25.63＆#177;0.58、21.63＆#177;0.58、17.59＆#177;1.29），差异有统计学意义（P〈0.05）。在动态组材料表面形成血管内皮细胞层完整，在植入犬右心房取出材料观察到血管内皮细胞层完整、细胞均匀分布，在静态组材料表面形成血管内皮细胞层不完整，在植入犬右心房取出材料观察到犬血管内皮细胞层不完整、细胞分布不均匀，胶原包埋羟基磷灰石涂层人工机械瓣膜有心脏血管内皮细胞附着在薄膜表面，单纯人工机械瓣薄膜表面大Objective To investigate the in-vivo re-cellularization of scaffold materials of artificial mechanical valves coated with collagen-embedded hydroxyapatite（HA）by comparing the in vitro and in vivo endothelialization,and to verify the performance of the scaffolds comprehensively. Methods Artificial mechanical valves coated with collagen-embedded HA were inoculated with cell suspension prepared from passage culture of canine vascular endothelial cells, and cultured in incubator. Then, the artificial mechanical valves coated with collagen-embedded HA in the static group were statically cultured in a 37℃incubator for 2 weeks,and those in the dynamic group were cultured in a specific culturing device with＆amp;nbsp;dynamic rotations for 2 weeks. The levels of nitric oxide （NO） and prostacyclin （PGI2） secreted by the vascular endothelial cells were determined. Uncoated valves（simple artificial mechanical valve group）,un-cultured valves（collagen-embedded HA-coated artificial mechanical valve group）,and valves in the static group and dynamic group were implanted in canine right atriums,respectively. Then,the valve materials were removed at the 6 weeks after the operation. Under scanning electron microscope,the cell adherence on the valve materials,in vivo re-cellularization of the scaffold materials,formation of endothelium layer,and performance of anticoagulation were determined. Results At 3,6,7,and 12d,the NO levels（110.92 ＆#177; 6.92,116.62＆#177;6.84,119.45＆#177;5.14,123.52＆#177;4.93）and PGI2 levels（19.21＆#177;0.52,29.13＆#177;0.57,26.67＆#177;0.52, 21.58 ＆#177; 1.34）secreted by vascular endothelial cells in the dynamic group were significantly higher than the NO levels（81.91＆#177;6.89,81.90＆#177;6.88,83.48＆#177;5.91,84.68＆#177;4.81）and PGI2 levels（16.31＆#177;0.55,25.63＆#177;0.58, 21.63 ＆#177; 0.58,17.59 ＆#177; 1.29） in the static group,with statistically significant differences （P〈0.05）. In the dynamic group,complete vascular endothelium layer

关 键 词：心脏瓣膜 人工 羟基磷灰石类 组织工程 

分 类 号：R318.08[医药卫生—生物医学工程]