山西省腹泻病原菌检测分析及耐药性研究  

Analysis of distribution and antimicrobial resistance of enteric pathogens in Shanxi Province

作　　者：冯军军[1] 姚素霞[2] 

机构地区：[1] 山西医学科学院山西大医院检,山西省太原030032 [2]山西省疾病预防控制中心疾病检验科

出　　处：《中国基层医药》2015年第16期2441-2443,共3页Chinese Journal of Primary Medicine and Pharmacy

摘　　要：目的：了解山西省感染性腹泻的细菌病原学分布特征及耐药性，以指导其临床用药。方法采集798例感染性腹泻患者粪便标本，分离可疑病原菌，用聚合酶链技术（PCR）、生化试验、血清凝集试验等方法鉴定可疑病原菌，分析细菌病原学分布特征；采用纸片扩增法，对分离到的病原菌进行药敏试验。结果798份标本分离各种肠道致病菌81株，阳性检出率10．15％。其中，致泻性大肠埃希菌检出最多，为46.91％（38／81），其次是志贺菌、沙门菌、气单胞菌等；对上述菌株进行纸片法药敏试验，大部分菌株对头孢西丁、头孢噻肟及环丙沙星的耐药性较低，而对四环素、萘啶酸的耐药性较高。结论山西省感染性腹泻的主要致病菌是致泻性大肠埃希菌、志贺菌、沙门菌和气单胞菌，应加强对这些菌的耐药性监测，为临床抗感染治疗提供可靠依据。Objective To investigate the distribution and antibiotic resistance of the enteric pathogens in Shanxi province to guide the choice of antibacterials.Methods 798 cases of fecal samples from patients with diarrhea were treated in the outpatient and inpatient.The suspected pathogen was separated then PCR,biochemical test,serum agglutination tests were used for identification of suspected pathogen,and bacterial pathogens constitution and patho-genic characteristics were analyzed.Antimicrobial susceptibility testing was conducted by diskdiffusion method for the suspected pathogen with 6 antimicrobial agents.Results 81 strains isolated from 798 specimens were positive with 10.15% for pathogen detection.Diarrheagenic Escherichia coli was the first frequently pathogen,accounting for 46.91%,followed by Shigella,Salmonella and Aeromonas.Drug sensitivity monitoring showed that the most of the 81 strains had lower level of resistance to cefoitin,cefotaxime and ciorofloxacin,and higher level of resistance to tetra-cycline and nalidixic aid.Conclusion Diarrheagenic Escherichia coli,Shigella,Salmonella and Aeromonas are the major bacterial diarrheal pathogens in the hospital.Surveillance of antimicrobial resistance in these bacteria should be strengthened to provide reliable evidence for clinical anti infection treatment.

关 键 词：腹泻 细菌病原学 耐药性 

分 类 号：R446.5[医药卫生—诊断学]