2种荧光定量PCR技术在HBV检测中的应用评价  

Evaluation of the two fluorescent quantitative PCR application in the detection of hepatitis B virus

作　　者：苟建军[1] 李远[1] 闫虹[1] 曹在秋 代志峰[1] 郭小兵[1] 

机构地区：[1]郑州大学第一附属医院检验科,河南郑州450000

出　　处：《中国卫生检验杂志》2015年第15期2500-2501,共2页Chinese Journal of Health Laboratory Technology

基　　金：河南省医学科技攻关计划项目(201303010)

摘　　要：目的探讨普通荧光定量PCR技术与内标法荧光定量PCR技术在乙型肝炎病毒(HBV)检测中的应用评价。方法采集郑州大学第一附属医院2013年6月-2014年6月HBV患者的血浆112份,分别用上述方法进行检测。配对卡方检验比较阳性检出率;观察普通法检测<500 IU/ml者的内标法结果;应用配对资料秩和检验正态近似法分析阳性结果差异。结果 112例标本中,内标法阳性率为77%(86/112);普通法阳性率为33%(37/112),两者差异具有统计学意义(P>0.05);37例普通法>500 IU/ml的标本,内标法结果均为阳性;75例普通法<500 IU/ml的标本,内标法结果26例为阴性,49例为阳性,其中23例<20 IU/ml,21例为20 IU/ml^300 IU/ml;2种方法检测HBV病毒阳性标本共37例,病毒载量统计分析差异无统计学意义(Z=0.717,P>0.05)。结论 2种方法均可用于活动期或高复制阶段患者检测对低复制阶段患者检测、治疗效果评估及预后监测,使用内标法更为合理。Objective To evaluate the application of common fluorescence quantitative PCR technology and internal standard method of fluorescent quantitative PCR in the detection of HBV. Methods Plasma of 112 cases of patients with HBV from the first affiliated hospital of Zhengzhou university from June 2013 to June 2014 was collected and was tested by the above methods.The positive rate of two methods with chi- square statistics was compared; the results of the internal standard method for detecting the 500 IU / ml by common observing method was observed; the difference of positive results was analyzed by paired data rank sum test normal approximation method. Results Of the 112 cases of samples,internal standard method positive rate was77%（ 86 /112）,general method positive rate was 33%（ 37 /112）,and the difference had statistically significant（P〈0. 05）. Of the 37 cases of samples whose values were more than 500 IU / ml detected by general method,internal standard method detection were all positive. Of the 75 cases of samples whose values detected by gerenal method were less than 500 IU / ml,while detected by internal standard method,26 cases were negative,49 cases were positive; 23 cases of viral load was less than 20 IU / ml,21 cases of loads between were 20 IU / ml- 300 IU / ml. A total of 37 cases of HBV virus positive samples were detected by the two methods and had no statistically difference（ Z = 0. 717,P〈0. 05）. Conclusion Two kinds of detection method can be used for patients with high activity or virus replication phase detection,but it is more reasonable to treat patients with lower replication phase detection,evaluation and prognosis monitoring.

关 键 词：聚合酶链式反应 病毒载量 乙型肝炎病毒 

分 类 号：R373.21[医药卫生—病原生物学]