合肥地区慢性无症状乙肝病毒携带者HBV s基因变异研究  

Study on the HBV s gene mutation in chronic asymptomatic hepatitis B virus carriers in Hefei

作　　者：张文艳[1] 李明[2] 汤仁树[1] 桂云[1] 金晶[1] 马尔健[1] 

机构地区：[1]合肥市疾病预防控制中心安徽省预防医学和公共卫生分子生物学检测重点实验室,安徽合肥230022 [2]安徽省肿瘤医院,安徽合肥230039

出　　处：《中国卫生检验杂志》2015年第15期2553-2555,共3页Chinese Journal of Health Laboratory Technology

基　　金：2011年省预防医学与公共卫生科研课题(2011Y0118)

摘　　要：目的了解合肥地区慢性无症状乙肝病毒携带者(As C)体内HBV s基因的变异情况。方法自慢性肝炎患者血清中提取HBV基因组DNA,扩增其s基因片段并进行序列分析,用MEGA 3.1和Clustalx 1.8软件为所测基因进行分型,并通过比对s基因序列,分析s基因变异情况。结果 27例样品中,B型13例(48.1%),C型14例(51.9%),未发现混合型和其他基因型。有12例(44.4%)标本s基因发生突变,其中B型6例,C型6例;单位点突变有8例,多位点突变有4例,其中B型有2例在氨基酸133位点发生突变(Met→Leu)。结论 27例慢性无症状乙肝病毒携带者HBV的基因型为B型和C型,As C患者体内HBV病毒s基因存在突变现象,并且突变位点随机,突变形式有单点突变和多位点突变。Objective To investigate the HBV s gene mutations of chronic asymptomatic hepatitis B virus carriers（ As C） in Hefei area. Methods HBV genomic DNA was extracted from serum of patients with chronic hepatitis,s genes were amplicated and performed sequence analysis and the tested genes were genotyped by MEGA 3. 1 and Clustalx 1. 8 and s gene mutation was analyzed by comparing s gene sequence. Results Of all the 27 cases,13（ 48. 1%） were genotype B,14（ 51. 9%） were genotype C,and mixed type and other gene types were not found. There were 12 cases（ 44. 4%） of s gene mutations,including 6cases of type B,6 cases of C type,8 cases of unit point mutation,and 4 cases of multiple point mutations,and among them two type B cases occurred mutation at the 133 amino acid sites from Met to Leu. Conclusion HBV genotypes of 27 cases of chronic asymptomatic hepatitis B virus carriers were type; HBV virus genes in patients with As C have gene mutations,the mutation site was random with the single point mutation and multiple point mutation.

关 键 词：乙型肝炎病毒 S基因 慢性乙型肝炎患者 

分 类 号：R512.62[医药卫生—内科学]