磁液悬浮离心式心室辅助装置实验研究  

Experimental research on magnetic and hydrodynamic suspension-centrifugal ventricular auxiliary device

作　　者：刘天文[1] 张杰民[1] 刘志刚[1] 刘晓程[1] 

机构地区：[1]泰达国际心血管病医院心血管外科,天津300457

出　　处：《中华生物医学工程杂志》2015年第3期242-246,共5页Chinese Journal of Biomedical Engineering

摘　　要：目的 应用体外模拟循环实验装置,对磁液悬浮离心式心室辅助装置（以下简称血泵）进行评估.通过动物实验,验证血泵的性能及可靠性.对血泵持续优化、改进.方法 取新鲜羊血500 ml,3.8％枸橼酸钠1∶9抗凝.固定转速下,调节流出道管径,使流出道和流入道压力差值在100±2mmHg.取转泵前和转后1、2、3、4h血样标本,测量其游离血红蛋白（Free Hemoglobin,FHB）含量.应用非体外循环、不停跳方法,将血泵入口植入绵羊左心尖,出口与降主动脉吻合.记录手术前后血泵的参数及绵羊的相关生化、血液指标.结果 体外实验中,血泵运行平稳,温升≤2.6℃.测得国际标准溶血指数（Normalized Index of Hemolysis,NIH）为0.002～0.006 g/100 L.在体动物实验,血泵辅助流量60％,血浆中游离血红蛋白＜45 mg/L.结论 经改进的血泵,整体参数得到优化,满足心室辅助需求.在体实验技术成熟,可开展大样本临床前动物实验.Objective To evaluate the magnetic and hydrodynamic suspension-centrifugal ventricular auxiliary device （blood pump） by using stimulated circulating experimental device in vitro,and to verify the performance and reliability of the blood pump in animal experiment,for continual optimization and improvement of the device.Methods 500 ml fresh sheep blood was obtained,added with 3.8% sodium citrate in a volume ratio of 1：9 for anticoagulation.Under the fixed rotation speed of the blood pump,the pipe diameter of the outflow tract was regulated to maintain the outflow and inflow tract pressure difference at 100± 2 mmHg.Before and after the rotation in the blood pump at 1,2,3 and 4 hours,the blood samples were obtained and the free hemoglobin （FHB） contents were measured.Using off-pump method,the blood pump inlet was implanted into the left cardiac apex of the sheep,and the outlet was sutured with the descending aorta.The blood pump parameters,and the biochemical parameters and blood parameters before and after surgery in the sheep were recorded.Results In the in vitro experiment,the blood pump operated steadily,the temperature rise ≤2.6 ℃,and the Normalized Index of Hemolysis （NIH） was 0.002 ～ 0.006 g/100L.In the in vivo animal experiment,the blood pump flow was 60%,and the plasma FHB〈45 mg/L.The seventh generation of the blood pump achieved great improvement.Conclusion In the in vitro experiment,the blood pump operated steadily,the temperature rise ≤2.6 ℃,and the Normalized Index of Hemolysis （NIH） was 0.002～0.006 g/100L.In the in vivo animal experiment,the blood pump flow was 60%,and the plasma FHB〈45 mg/L.The seventh generation of the blood pump achieved great improvement.

关 键 词：左心辅助装置 心力衰竭 游离血红蛋白 

分 类 号：R654.2[医药卫生—外科学]