正常产妇羊水、血浆蛋白质指纹图谱分析的研究  

Analysis of fingerprint patterns of amniotic fluid and plasma proteins in healthy pregnant women

作　　者：朱斌[1] 周坚[1] 陈芳[1] 黎丽红 叶志海 夏鲁全 

机构地区：[1]南方医科大学附属何贤纪念医院妇产科,广州511400 [2]百奥生物工程有限公司,珠海519080

出　　处：《新医学》2015年第8期507-510,共4页Journal of New Medicine

基　　金：广东省科技厅社会发展领域科技计划项目(2010B031600179)

摘　　要：目的比对羊水、血浆蛋白质指纹图谱,寻找羊水特异性表达蛋白,为产科学的临床研究提供分子依据。方法应用表面增强激光解吸电离飞行时间质谱仪采用4种不同原理的蛋白芯片检测了12名正常足月产妇的羊水、血浆蛋白质指纹图谱。结果羊水和血浆标本分别测得蛋白信号(信噪比大于3)150个和250个。4种芯片检出羊水特异性蛋白30个,相对分子质量为11 198、12705 Da的蛋白是比较确定的羊水特异性蛋白。结论羊水中存在特异性表达蛋白,其在妊娠相关生理、病理学中可能起重要作用。Objective To explore the proteins specifically-expressed in the amniotic fluid（ AF） by comparing the fingerprint patterns of in the AF and plasma proteins,aiming to provide molecular evidence for obstetrics research. Methods Surface enhanced laser desorption / ionization time-of-flight mass spectrometry（ SELDI-TOF-MS） with four different protein-chips was utilized to investigate the fingerprint patterns of AF and plasma proteins from 12 healthy full-term pregnant women. Results In total,150 protein signals（ signal-tonoise ratio〉 3） were detected in the AF and 250 in the plasma. Thirty proteins were detected specifically expressed in the AF using four types of protein-chips. The proteins with a molecular weight of 11198 Da and12705 Da were confirmed to be specifically expressed in the AF. Conclusion The AF contains specificallyexpressed proteins,which probably play a vital role in the physiological and pathological progresses during pregnancy.

关 键 词：羊水 血浆 蛋白质指纹图谱 

分 类 号：R714.1[医药卫生—妇产科学] R446.1[医药卫生—临床医学]