精脒对Caco-2细胞生长及摄取葡萄糖能力的影响  

Effect of spermidine on Caco-2 cell growth and glucose uptake

作　　者：陈婷[1] 王桂香[2] 潘雪刁[2] 王晶[1] 黎同明[1] 

机构地区：[1]广州中医药大学中药学院,广东广州510006 [2]广东药学院药科学院,广东广州510006

出　　处：《广东药学院学报》2015年第4期500-503,共4页Academic Journal of Guangdong College of Pharmacy

基　　金：国家自然科学基金项目(81302895)

摘　　要：目的研究精脒(SPD)及多胺合成特异性抑制剂二氟甲基鸟氨酸(DFMO)对Caco-2细胞生长及摄取葡萄糖能力的影响。方法取对数生长期结肠癌Caco-2细胞,分为对照组、DFMO组和SPD+DFMO组进行培养,MTT法检测细胞的生长,葡萄糖测定试剂盒和荧光标记2-脱氧葡萄糖(2-NBDG)检测Caco-2细胞葡萄糖摄取和消耗的情况,Western blot检测DFMO和SPD+DFMO对Caco-2细胞葡萄糖转运蛋白1(GLUT1)表达的影响。结果 DFMO作用24、48、72 h对细胞生长有明显抑制作用,SPD+DFMO组对Caco-2细胞生长有促进作用,同时SPD能够改善DFMO抑制Caco-2细胞摄取葡萄糖的作用,荧光显微镜下观察到SPD+DFMO组荧光强度增强,Western blot结果显示,DFMO可抑制GLUT1蛋白的表达,SPD+DFMO组Caco-2细胞GLUT1蛋白随着SPD浓度升高表达增加。结论 SPD在一定浓度范围内可以逆转DFMO引起的Caco-2细胞增殖的抑制,促进葡萄糖摄取。Objective To study the effect of spermidine（ SPD） and polyamine synthesis inhibitor DFMO on Caco-2 cell growth and glucose uptake. Methods Caco-2 cells on the logarithmic phase were cultivated with SPD and DFMO. The cell growth was determined by MTT,the glucose consumption was detected by fluorescence-labeled 2-deoxidation of the glucose（ 2-NBDG） and glucose test kit,and the expression of GLUT1 was detected by western blot. Results DFMO obviously inhibited the cell proliferation at 24 h,48 h and 72 h.The SPD combined with DFMO group stimulated cell growth. SPD optimization improved DFMO restrain on Caco-2 cell uptake of glucose. Fluorescence intensity enhanced in the SPD combined with DFMO group. The expression of GLUT1 protein increased along with the SPD concentration. Conclusion SPD can stimulate Caco-2 cell growth restrained by DFMO in a certain concentration range,and promote the glucose uptake.

关 键 词：精脒 二氟甲基鸟氨酸 CACO-2细胞 葡萄糖摄取 

分 类 号：R96[医药卫生—药理学]