罗布麻通过抑制氧化应激反应减轻心肌缺血再灌注损伤  

Apocynum Venetum Attenuates Myocardial Ischemia/reperfusion Injury via Inhibiting Oxidative Stress

作　　者：屈小玲[1,2] 王文清[3] 梁向艳[1] 铁茹[1] 刘芳娥[1] 李榕[4] 张海锋[1] 

机构地区：[1]第四军医大学教学实验中心,陕西西安710032 [2]第四军医大学校直门诊部,陕西西安710032 [3]第四军医大学唐都医院血液科,陕西西安710038 [4]第四军医大学西京医院老年病科,陕西西安710032

出　　处：《现代生物医学进展》2015年第19期3609-3612,共4页Progress in Modern Biomedicine

基　　金：国家自然科学基金项目(81270330;81470413;81300190);陕西省科学技术研究发展计划项目(2013KJXX-89)

摘　　要：目的:观察罗布麻叶提取物(apocynum venetum leaf extract AVLE)预处理对心肌缺血再灌注(MI/R)损伤的影响。方法:采用SD大鼠MI/R模型,随机分为sham(假手术)组、MI/R组、AVLE预处理+MI/R组,检测血流动力学,采用氯化三苯基四氮唑和伊文思蓝双染法检测心梗面积、以血浆肌酸激酶(CK)和乳酸脱氢酶(LDH)活性检测心肌损伤情况、以超氧化物、丙二醛(MAD)和超氧化物歧化酶(SOD)含量检测心肌氧化应激以及Western blot方法检测gp91phox的表达。结果:与MI/R组相比,AVLE预处理组左室压上升、下降最大速率(±LVdp/dtmax)升高(P<0.05),心肌梗死面积减少,两组分别为41.5±4.5%和32.0±3.5%(P<0.05),血浆CK和LDH活性分别降低到1653±62 U/L和2461±152 U/L(P<0.05),减少了心肌组织超氧化物的含量(P<0.05)。AVLE治疗显着降低gp91phox的表达(P<0.05),使SOD活性增加(P<0.05),MDA水平显著降低(P<0.05)。结论:AVLE通过抑制I/R心肌的氧化应激发挥心脏保护作用。Objective： To observe the effects of AVLE preconditioning on myocardial ischemia/reperfusion （MI/R） injury. Methods： Male Sprague-Dawley rats were divided into 3 groups randomly： sham, ischemia reperfusion and AVLE preconditioned group. The model of myocardial I/R injury in vivo was made by ligating the left anterior descending artery for 30 min followed by 4 h of reperfusion in SD rats. The hemodynamics were measured. The myocardial infarct size was measured by Evans blue and 2,3,5-triphenyltetrazolium chloride （TTC） staining. The level ofhyperoxide superoxide, malondialdehyde （MDA） and plasma creatine kinase （CK） were measured. The activities of ant^oxidant enzyme superoxide dismutase （SOD） and lactate dehydrogenase （LDH） were detected. The gp91~ were determined by Western Blot. Results： Compared with I/R group, the left ventricular ＋ dp/dtmax in AVLE preconditioned rats was increased （P〈0.05）. The percentage of area of necrosis was reduced （32.0±3.5% vs. 41.5±4.5%, P〈0.05）. The plasma CK and LDH levels were decreased to 1653±62 U/L and 2461 ±152 U/L （P〈0.05 vs. MI/R group）. AVLE preconditioning decreased superoxide accumulation （P〈0.05 vs. MI/R group）. AVLE treatment markedly decreased gp91~ expression （P〈0.05 vs. MI/R group）. The activity of SOD was increased （P〈0.05 vs. MUR group）. The level of MDA was decreased （P〈0.05 vs. MI/R group）. Conclusions： AVLE alleviates UR injury in myocardium by inhibition of excessive ERS.

关 键 词：罗布麻 氧化性应激 心肌缺血再灌注损伤 

分 类 号：Q95-3[生物学—动物学] R541[医药卫生—心血管疾病]