自体软骨细胞修复兔膝关节全层软骨缺损的实验研究  

Experimental Study of Autologous Chondrocyte Repair of Rabbit Full-thickness Cartilage Defects of the Knee

作　　者：申福国 孔维丽[1] 吕涵宁 武广海 崔宏宇[1] 刘士臣[1] 赵鹏[1] 郭全义[2] 杨建华[1] 

机构地区：[1]佳木斯大学附属第一医院,黑龙江佳木斯154003 [2]解放军总医院骨科研究所,北京100853

出　　处：《现代生物医学进展》2015年第20期3839-3842,共4页Progress in Modern Biomedicine

基　　金：佳木斯大学研究生科技创新项目（LZR2014_022）;国家级大学生创新创业训练项目（1110150221）

摘　　要：目的：研究自体软骨细胞复合于人脐带Wharton胶支架对兔膝关节全层软骨缺损的修复效果。方法：经自体关节软骨细胞经体外培养后复合到制备人脐带Wharton胶取向支架内构建细胞-支架复合体，选取健康清洁新西兰兔23只，雌雄不拘，体重2．5．3．0kg，取滑车沟中下部制作全层软骨缺损模型后随机分成A、B和C组。A组（n=10）：植入自体软骨细胞＋人脐带Wharton胶取向支架复合体；B组（n=10）：植入单纯人脐带Wharton胶取向支架；C组（n=3）：不做任何处理正常兔。分别于术后3个月和6个月各处死后取材进行生物力学特性评估检测。结果：压痕实验显示在3个月时A和B组修复区组织刚度分别达到正常软骨的45．72％和25．25％。且A组刚度明显优于B组，均低于C组（P〈0．05）；到6个月时各自达到正常软骨刚度的69．76％和35．14％，同样A组刚度明显优于B组，均低于C组（P〈0．05）且在同期个各组之间均有显著性差异（F=80．309，P〈0．05）。结论：体外培养的自体软骨细胞与人脐带Wharton胶复合在体内的微环境作用下修复软骨缺损效果良好。为软骨组织工程提供了一种新支架材料。Objective： To investigate the effect of autologous chondrocytes in human umbilical cord Wharton＇s jelly matrix scaffold stents on rabbit knee full thickness cartilage defect repair. Methods： Autologous chondrocyte after in vitro preparation of the composite to build cells from human umbilical cord Wharton＇s jelly matrix glue oriented scaffolds - stents complex, choose healthy and clean 23 rabbits, male or female, weighing 2.5-3.0 kg, take the lower pulley groove were randomly divided into A, B and C after making a full thickness cartilage defect model. Group A （n=10）： autologous chondrocyte implantation＋human umbilical cord Wharton＇s jelly matrix plastic composite scaffold orientation; group B （n=10）： implanted human umbilical cord Wharton＇s jelly malaix simple plastic bracket orientation; group C （n=3）： no process for normal rabbit. Were everywhere after 3 months and 6 months after the death of biomechanical properties derived assessment test. Results： The indentation experiments are displayed in three months organizing stiffness restoration area A and B, raspoctively 45.72% and 25.25% of normal cartilage and compare with group A, the stiffness was significantly better than in group B, and both were lower than in group C （P〈0.05）; to 6 months when each reached 35.14% and 69.76% of normal cartilage stiffness, compare with group A, the stiffness was significantly better than in group B, and both were lower than in group C （P 〈0.05） and in the same period of months between the groups was significant difference （F=80.309, P〈0.05）. Conclusion： Autologous cultured chondrocytos and human umbilical cord Wharton＇s jelly matrix scaffold composite repair cartilage defects to good effect in rive microenvironment for cartilage tissue engineering provides a new scaffold.

关 键 词：人脐带Wharton胶 支架 软骨细胞 软骨缺损 

分 类 号：Q95-3[生物学—动物学] R68[医药卫生—骨科学]