神经干细胞移植对AD模型大鼠SNAP-25表达的影响  

Effect of Neural Stem Cell Transplantation on SNAP-25 of Alzheimer's Disease Rats

作　　者：杜红蕾[1] 宋春雨[1] 王泽宇[2] 魏春杰[2] 韩凤[2] 杨晓玉[2] 盛宝英[2] 

机构地区：[1]佳木斯大学,黑龙江佳木斯154002 [2]佳木斯大学附属第一医院神经内科,黑龙江佳木斯154002

出　　处：《现代生物医学进展》2015年第21期4020-4024,共5页Progress in Modern Biomedicine

基　　金：黑龙江省教育厅科学技术研究项目(12531698);黑龙江省高校科技创新团队项目(2012TD013);佳木斯大学研究生创新基金(LZR2014_018)

摘　　要：目的:观察神经干细胞对AD大鼠海马周围微环境中SNAP-25表达及其认知功能的影响。方法:取成年雄性Wistar大鼠30只,随机分为对照组、AD模型组、细胞移植组,每组10只。采用凝聚态Aβ1-42注射到大鼠海马组织内建立阿尔茨海默病(AD)大鼠动物模型,通过Y迷宫测试大鼠学习记忆能力和Western blot技术检测大鼠海马组织内SNAP-25的表达。结果:Y迷宫测试结果显示术后4周时AD模型组和细胞移植组大鼠学习记忆均低于对照组,与AD模型组比较,细胞移植组大鼠学习记忆能力明显高于AD模型组,差异有统计学意义(P<0.05);Western blot检测结果显示术后4周时AD模型组和细胞移植组大鼠海马组织内SNAP-25蛋白表达量均低于对照组,与AD模型组比较,细胞移植组大鼠海马组织SNAP-25蛋白表达量高于AD模型组差异有统计学意义(P<0.05)。结论:移植的NSCs可改善AD大鼠的学习和记忆能力,其机制可能是通过改变海马区周围的微环境并上调了海马组织内SNAP-25表达。Objective： To investigate the effects of neural stem cells in rat hippocampal AD surrounding microenvironment SNAP-25 expression and cognitive function. Methods： 30 Adult male Wistar rats were randomly divided into control group, AD model group, cell transplantation groups of 10. Aβ1-42 injection to establish the use of condensed matter senile dementia （AD） in the hip- pocampus of rats rat model of learning and memory in rats by Y-maze test, SNAP-25 expression within the Western blot technique rat hippocampus. Results： Y maze test results show after 4 weeks when AD model group and cell transplantation group learning and memo- ry in rats were lower than the control group, compared with the AD model group, cell transplantation group learning and memory abili- ties of rats was significantly higher than AD model group, the difference statistically significant （P〈0.05）; Western blot test results show after 4 weeks AD model group and cell transplantation in the rat hippocampus SNAP-25 protein expression levels than the control group, compared with the AD model group, cell transplanted rat SNAP-25 protein expression in hippocampal tissues than AD model group, the difference was statistically significant （P〈0.05）. Conclusion： Transplanted neural stem cells can improve learning and memory in AD rats, and its mechanism may be by altering the microenvironment surrounding the hippocampus and raised the SNAP 25- expression within the hippocampus.

关 键 词：神经干细胞 移植 阿尔茨海默病 SNAP-25 

分 类 号：Q421[生物学—神经生物学] Q95-3[生物学—生理学]