人Daintain基因5'调控区序列的生物信息学分析  

Bioinformatics Analysis of the Human Daintain Gene 5' Regulatory Region

作　　者：颜冬菁[1,2] 王青松[1] 唐敏[3] 王咸寿 冯秋芳[3] 陈正望[2] 

机构地区：[1]海南医学院理学院生化与分子生物学教研室,海南海口571199 [2]华中科技大学生命科学与技术学院,湖北武汉430074 [3]海南医学院理学院生物技术专业实验室,海南海口571199

出　　处：《现代生物医学进展》2015年第21期4178-4181,共4页Progress in Modern Biomedicine

基　　金：国家自然科学基金项目(81460558;81172511);海南医学院人才引进科研启动经费(2013005)

摘　　要：目的：探讨人Daintain基因5’调控区的序列特征。方法：利用在线软件BLAST、Neural Network Promoter Prediction、Promoter2．0、Promoter SCAN、EMBOSS、CpG Island Searcher和TFSEARCH预测人Daintain基因启动子区域、CpG岛分布和转录因子结合位点。结果：人Daintain基因5’调控区存在1个CAAT盒。Daintain基因可能存在6个启动子位点，CpG岛可能位于216bp区间（23～238bp）。评分85分以上时，该序列存在251个可能的转录因子结合位点；评分90分以上时，该序列存在70个可能的转录因子结合位点；评分95分以上时，该序列存在16个可能的转录因子结合位点；评分100分以上时，该序列存在7个可能的转录因子结合位点；这些结合的转录因子基本是与免疫细胞增殖或性别发生有关。结论：人Daintain基因5’调控区的生物信息学研究表明其转录受甲基化和多种转录因子的调控，为研究Daintain基因启动子的功能提供理论基础。Objective： To investigate the sequence characteristics of human Daintain gene 5＇ regulatory region. Methods： The pro- moter region, distribution of CpG island and transcriptional binding sites of human Daintain gene were predicted by online software such as BLAST, Neural Network Promoter Prediction, Promoter 2.0, Promoter SCAN, EMBOSS, CpG Island Searcher and TF SEARCH. Results： The 5＇ regulatory region of Daintain gene contained a CAAT box. The Daintain gene may contained 6 promoter regions, a 216 bp （23 - 238 bp） CpG island. 251 potential transcription factors binding sites were predicted in the 5＇ regulatory region of Daintain gene when the score higher than 85 scores; 70 potential transcription factors binding sites were predicted when the score higher than 90 scores; 16 potential transcription factors binding sites were predicted when the score higher than 95 scores; 7 potential transcription factors bind- ing sites were predicted when the score higher than 100 scores. A majority of potential transcription factors were associated with im- munocyte proliferation and sex determination. Conclusion： Research by bioinformatics technology suggested that the transcription of Daintain gene was regulated by DNA methylation and many transcription factors, and provided information for the study on the promoter of Daintain gene.

关 键 词：人Daintain基因 5’调控区 启动子 CPG岛 转录因子结合位点 

分 类 号：R34[医药卫生—基础医学] Q756[生物学—分子生物学]