Detection and fine-mapping of SC7 resistance genes via linkage and association analysis in soybean  

作　　者：Honglang Yan Hui Wang Hao Cheng Zhenbin Hu Shanshan Chu Guozheng Zhang Deyue Yu 

机构地区：[1]National Center for Soybean Improvement, National Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University [2]Kunming Institute of Botany, Chinese Academy of Sciences

出　　处：《Journal of Integrative Plant Biology》2015年第8期722-729,共8页植物学报（英文版）

基　　金：supported in part by the National Basic Research Program of China(973 Program)(2010CB125906);the National Natural Science Foundation of China(31171573,31301342,31370034);the Jiangsu Provincial Support Program(BE2012328);the Program for Changjiang Scholars;the Innovative Research Team in University(PCSIRT13073)

摘　　要：Soybean mosaic virus（SMV） disease is one of the most serious and broadly distributed soybean（Glycine max（L.） Merr.） diseases. Here, we combine the advantages of association and linkage analysis to identify and fine-map the soybean genes associated with resistance to SMV strain SC7.A set of 191 soybean accessions from different geographic origins and 184 recombinant inbred lines（RILs） derived from Kefeng No.1（resistant） Nannong 1138-2（susceptible） were used in this study. The SC7 resistance genes were previously mapped to a 2.65 Mb region on chromosome 2 and a 380 kb region on chromosome 13. Among 19 single nucleotide polymorphisms（SNPs） detected via association analysis in the study, the SNP BARC-021625-04157 was located in the2.65 Mb region, and the SNP BARC-041671-08065 was located near the 380 kb region; three genes harboring the SNPs were probably related to SC7 resistance. The resistance gene associated with BARC-021625-04157 was then finemapped to a region of approximately 158 kb on chromosome2 using 184 RILs. Among the 15 genes within this region, one NBS-LRR type gene, one HSP40 gene and one serine carboxypeptidase-type gene might be candidate SC7 resistance genes. These results will be useful for map-based cloning and marker-assisted selection in soybean breeding programs.Soybean mosaic virus（SMV） disease is one of the most serious and broadly distributed soybean（Glycine max（L.） Merr.） diseases. Here, we combine the advantages of association and linkage analysis to identify and fine-map the soybean genes associated with resistance to SMV strain SC7.A set of 191 soybean accessions from different geographic origins and 184 recombinant inbred lines（RILs） derived from Kefeng No.1（resistant） Nannong 1138-2（susceptible） were used in this study. The SC7 resistance genes were previously mapped to a 2.65 Mb region on chromosome 2 and a 380 kb region on chromosome 13. Among 19 single nucleotide polymorphisms（SNPs） detected via association analysis in the study, the SNP BARC-021625-04157 was located in the2.65 Mb region, and the SNP BARC-041671-08065 was located near the 380 kb region; three genes harboring the SNPs were probably related to SC7 resistance. The resistance gene associated with BARC-021625-04157 was then finemapped to a region of approximately 158 kb on chromosome2 using 184 RILs. Among the 15 genes within this region, one NBS-LRR type gene, one HSP40 gene and one serine carboxypeptidase-type gene might be candidate SC7 resistance genes. These results will be useful for map-based cloning and marker-assisted selection in soybean breeding programs.

关 键 词：Association analysis FINE-MAPPING linkage analysis SOYBEAN soybean mosaic virus 

分 类 号：Q943.2[生物学—植物学]