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作 者:徐健程 黄海裙 张源生 王晓维[1] 许冬华 黄国勤[1]
机构地区:[1]江西农业大学生态科学研究中心,江西南昌330045 [2]井冈山大学生命科学学院,江西吉安343009 [3]井冈山应用科技学校,江西吉安343001
出 处:《广东农业科学》2015年第16期12-18,共7页Guangdong Agricultural Sciences
基 金:江西省高校科技落地计划项目(KJLD13067)
摘 要:通过盆栽试验,设置3个干旱胁迫处理(对照、轻度胁迫、重度胁迫),研究干旱胁迫和复水对金沙柚叶片抗氧化酶活性及丙二醛(MDA)含量等生理指标的影响。结果表明:干旱胁迫下,随着干旱胁迫程度的加强,超氧化物歧化酶(SOD)活性提高,过氧化氢酶(CAT)活性降低,MDA、脯氨酸、可溶性蛋白、可溶性糖的合成加快,叶片相对含水量降低。复水20d后,SOD、POD活性及可溶性糖含量先上升后下降,脯氨酸含量、可溶性蛋白含量、叶片相对含水量和CAT活性与干旱胁迫表现一致,而MDA含量则先下降后上升。各处理下生理指标均随着试验时间推移产生一定程度变化。各生理指标通过协同作用,为金沙柚应对干旱及旱涝急转环境变化起到保护作用。Pot experiments were carried out to study the effects of drought stress (light drought stress, heavy drought stress ) and rehydration on the activities of superoxide dismutase ( SOD ), catalase ( CAT ), peroxidase ( POD ), the content of malondialdehyde ( MDA ) and so on. The results showed that, with the drought stress level strengthening, the activity of SOD, the synthesis of MDA, soluble sugar content increased, the activity of CAT and leaf relative water content reduced. 20 days after rehydration SOD activity, POD activity and soluble protein content decreased after an initial increasing; proline content, soluble protein, leaf relative water were consistent with the degree of drought stress, while the MDA content decreased at first and then increased. Each physiological and physiochemical index played a protective role in Jinshayou' s response to the dramatic change of drought and flood environment.
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