KDR基因多态性的杰出有氧耐力表型与运动应激诱导的循环microRNA差异表达谱的关联研究  

Relationship of Elite Endurance Phenotype of KDR Gene Polymorphism with Exercise Induced Circulating MicroRNA Profile

作　　者：许英樱[1] 段立公[2] 徐思[3] 

机构地区：[1]四川师范大学体育学院,四川成都610100 [2]国家体育总局运动医学研究所体育医院,北京100061 [3]四川省医学科学院四川省人民医院,四川成都610031

出　　处：《天津体育学院学报》2015年第2期121-126,共6页Journal of Tianjin University of Sport

基　　金：国家科技攻关项目(项目编号:2005BA904)

摘　　要：目的:分析KDR基因SNP/rs1870377的A/A基因型的杰出有氧耐力表型与训练诱导的循环mi RNA表达特征的关联。方法:试验对象为33名现役国家级耐力项目男性运动员和58名体育教育专业男性大学生(二级运动员),均为汉族。递增负荷法检测最大摄氧量、个体乳酸阈;mi RNAarray及q RT-PCR检测训练诱导的c-mi RNA表达谱,Taqman法解析KDR基因SNP/rs1870377基因型,分析优势基因型及其杰出耐力表型与训练诱导的c-mi RNA表达特征的关联。结果:A/A基因型的杰出耐力表型差异表达17条c-mi RNA,9条上调,8条下调;mi RNA同步调控AMPK、p53、PPAR、MAPK、m TOR等信号通路多个基因表达。结论:差异表达的mi RNA的调控作用同步上调AMPK-PGC-1α通路和p53通路功能,促进线粒体合成和有氧代谢能力,在A/A基因型上调VEGF通路活性增强心肌/骨骼肌供血条件下进一步提高有氧代谢供能效率,对杰出耐力表型的形成具有促进作用;mi RNA的调控作用与rs1870377的A/A基因型的杰出有氧耐力表型存在关联,二者可作为预测有氧耐力发展潜力的表观遗传学分子标记,联合应用于运动选材。Objective：To explore the correlation between the Elite endurance phenotype of A/A genotype of KDR gene rs1870377 and the differential expres-sion profile of c-mi RNA induced by exercise. Method：33 active elite athletes（national grade）and 58 student athletes（grade 2）were engaged in this test.V·O2max and ILT were measured. rs1870377 of KDR gene and differential expression profile of c-mi RNAs induced by exercise were detected. Targetscan,mi-Randa,and mi RBase were used to predict target genes of those mi RNAs,and pathway analysis was performed for target gene by KEGG and IPA. Results：There were 17 c-mi RNAs which regulated the pathway of AMPK,p53,PPAR,MAPK,m TOR,and their regulating characters were related to the functionsof KDR enhanced by A/A genotype,which were validated to promote the formation of the elite endurance. Conclusions：Cooperated with rs1870377,the pro-file of c-mi RNAs induced by exercise can be used as a biomarker on forecasting and valuing the potential of aerobic endurance.

关 键 词：杰出有氧耐力表型 血管内皮生长因子受体2 单核苷酸多态性 运动应激 循环micro RNA 

分 类 号：G804.2[文化科学—运动人体科学]