玉米赤霉烯酮降解菌的分离与鉴定  

Isolation and identification of a bacterial strain capable of degrading zearalenone

作　　者：龙淼[1] 何润霞[1] 刘义[2] 关心[1] 王媛[1] 陈新亮[1] 郭佳怡[1] 高增贵[3] 

机构地区：[1]沈阳农业大学畜牧兽医学院,辽宁沈阳110866 [2]四川理工学院化学与制药工程学院,四川自贡643000 [3]沈阳农业大学植物保护学院,辽宁沈阳110866

出　　处：《畜牧与兽医》2015年第8期34-38,共5页Animal Husbandry & Veterinary Medicine

基　　金：国家自然科学基金项目(31201961;31302152);中国博士后科学基金资助项目(2014M551125);辽宁省教育厅科学研究一般项目(L2014561)

摘　　要：以被霉菌毒素污染的饲料、健康家畜粪便、土壤等为样本，采用玉米赤霉烯酮（ZEN）毒素为唯一碳源进行ZEN毒素降解菌的驯化富集培养，并用高效液相色谱法对分离到的细菌进行ZEN毒素降解能力检测。结果显示：共筛选到14株具有能够降解ZEN的菌株，降解率在8．3％～87．1％之间，其中从石油污染土地筛选到的菌株SYA13在72h对液体培养基中的ZEN毒素的降解能力达87．1％以上，明显高于其他菌株；对SYA13菌株从形态、生理生化特性以及16SrRNA序列进行分析，初步鉴定SYA13菌株为红球菌（Rhodococcus）。ZEN高效降解菌SYA13菌的分离鉴定，为继续研究其降解机制及实际应用奠定了基础。A mixed culture of bacteria, enriched from mycotoxins contaminated feed, soil and animal feces, proved capable of degrading zearalenone （ZEN） by high performance liquid chromatography （HPLC） method when ZEN was used as the only carbon source. A total of 14 strains capable of degrading ZEN were screened, and the degradation ratios of the 14 strains ranged from 8.3% to 87.1%. The degradation ratio of strain SYA13 was found to be highest and reached more than 87. 1% at 72h in the culture, which was obviously higher than oth- ers. According to its morphological, physiological, and biochemical characteristics and 16S rRNA gene sequence, the strain SYA13 was i- dentified as Rhodococcus. This study laid the foundation for investigation of degradation mechanism and actual application of SYA13.

关 键 词：玉米赤霉烯酮 降解 红球菌 生物解毒 

分 类 号：S852.6[农业科学—基础兽医学]